Earticle

초록

영어

Betulin, a pentacyclic triterpenoid, abundantly accumulated in Inonotus obliquus (chaga mushroom), exhibits strong anti-inflammatory, antioxidant, anticancer, and wound-healing properties. However, its extraction remains challenging due to its poor solubility and thermal sensitivity. In this study, we optimized ultrasound-assisted extraction (UAE) using response surface methodology (RSM) to maximize simultaneous betulin and antioxidant compound recovery from I. obliquus. We evaluated three extraction variables (i.e., time, temperature, and ethanol concentration) using a three-factor, five-level design. All quadratic models were significant (p < 0.05), with R² values ranging from 0.83 to 0.93 and prediction errors remaining below 5 %, thereby confirming strong model reliability. Multi-response optimization using a superimposed response plot identified 92.08 % ethanol, 42.56 min, and 62.19 °C as a narrow optimal region, in which all responses simultaneously met the desired criteria. Under these conditions, extraction was predicted to yield high phenolic content (2.58 mg GAE/g DM), increased flavonoid levels (0.57 mg QE/ g DM), strong DPPH radical-scavenging activity (87.49 % DSA), and a betulin content of 2.00 mg/g DM. In contrast, low ethanol concentrations, excessive heating, or prolonged extraction times resulted in reduced yields due to the oxidative or thermal degradation of the bioactive constituents. Overall, the optimized extraction conditions emphasize the importance of controlling solvent polarity and balancing the temperature and time parameters to prevent thermolabile compound decomposition. These results provide a reproducible and eco-efficient framework for large-scale antioxidant constituent extraction from I. obliquus.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
재료 및 추출
초음파 보조 추출공정
총 폴리페놀 및 플라보노이드 함량 분석
항산화 활성 평가
베툴린 정량 분석
반응표면분석법 설계
회귀모델 구축 및 통계적 평가
RESULTS AND DISCUSSION
모델 적합성 평가
추출조건이 총 폴리페놀 함량(TPC)에 미치는 영향
추출조건이 총 플라보노이드 함량(TFC)에 미치는 영향
추출조건이 항산화 활성에 미치는 영향
주요 생리활성 성분 분석
추출조건이 베툴린 추출에 미치는 영향
최적 추출조건 확인
CONCLUSION
REFERENCES

키워드

저자

• 한민호 [ Min Ho Han | 선문대학교 식품공학·영양학부 ]
• 오종현 [ Jong Hyun Oh | 선문대학교 식품공학·영양학부 ]
• 이주환 [ Ju Hwan Lee | 선문대학교 식품공학·영양학부 ]
• 김진우 [ Jin Woo Kim | 선문대학교 식품공학·영양학부/선문대학교 차세대 반도체 기술센터 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635