Oleander(Nerium oleander), which contains toxic substances of oleandrin, blooms with white, yellow, and pink flowers. In this study, we investigated how much the genes differ according to flower color within oleander species. A total of 14 oleander individuals were used, 10 individuals with known flower color(two of white flowers, one of yellow flowers, and seven of pink flowers) and 4 individuals with unknown flower color. The genetic markers used in this study are five chloroplast markers(psbA-trnH intergenic spacer(IGS), trnL-trnF IGS, trnL intron, rbcL, matK) and four nuclear markers(intergenic transcribed spacer region(ITS), 18S ribosomal RNA(rRNA), phenylalanine ammonia-lyase(PAL), xanthine dehydrogenase(Xdh)). As a result of analyzing nucleotide sequences of these genetic markers from 14 individuals, three chloroplast genetic markers of psbA-trnH IGS, trnL-trnF IGS, and trnL intron showed some differences and three markers of rbcL, matK, and 18S rRNA did not. For the ITS sequence, a total of 84 clones(6 clones each from 14 oleander samples) were obtained using the vector cloning method, resulting in 79 oleander ITS sequences, and the remaining 5 were fungal ITS sequences. Even within a single sample, diverse ITS sequences were observed. PAL and Xdh showed duplicated nucleotide sequences of Y, R, S, and W. Four samples of unknown flower color showed identity with individuals with pink(double) flowers in the nucleotide analysis. Distinct sequence variations based on flower color were not consistently observed across all genetic markers. The nucleotide sequences obtained from this study were registered into GenBank of NCBI. The genetic distance was the highest with the ITS marker, but based on the ease of the experiment, psbA-trnH IGS marker seems to be the best plant barcode in this study.
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Abstract Ⅰ. 서론 Ⅱ. 재료 및 방법 1. 협죽도 시료 및 DNA 분리 2. 유전자 마커와 염기서열 분석 Ⅲ. 결과 및 고찰 1. 엽록체 유전자 마커에 의한 분류 2. 핵내 유전자 마커에 의한 분류 Ⅳ. 결론 Ⅴ. 사사 Ⅵ. 참고문헌
법과학 분야는 사회정의 구현에 있어 크나큰 가치가 있음에도 불구하고 우리나라에서는 이 분야에 대한 인식이 미흡하여 선진 외국에 비해 침체되어 있는 실정이다. 이에 우리나라에서도 법과학 분야와 관련 있는 학계, 연구기관, 수사기관 등 유관 단체들로 구성된 한국 법과학회를 창립하여 이 분야를 활성화 시켜 과학수사를 한층 더 발전시키기 위함을 목적으로 한다.