Earticle

초록

영어

The medicinal fungus Cordyceps militaris is recognized for producing cordycepin, a bioactive nucleoside with anticancer, immunomodulatory, and antioxidant properties. However, conventional culture media often entail high production costs and limited sustainability, prompting the search for alternative nutrient sources. This study evaluated onion, green onion, and garlic peel extracts—agricultural by-products rich in flavonoids, phenolics, and sulfur-containing antioxidants—as sustainable substrates for enhancing mycelial biomass and cordycepin biosynthesis in C. militaris. Liquid cultures supplemented with peel extracts (1–5%) were assessed for growth, cordycepin production (HPLC), and antioxidant activity (DPPH assay). Onion peel extract (OPE) showed the strongest growth-promoting effect, yielding 8.2 g/L of biomass at 5% and achieving a 19% increase in cordycepin concentration at 3% compared with the control. Antioxidant activity strongly correlated with cordycepin accumulation (R = 0.96, p < 0.001), indicating that secondary metabolite production contributed significantly to radicalscavenging capacity. Response surface methodology using a Box–Behnken design revealed that extract concentration, pH, and incubation period significantly influenced cordycepin production (p < 0.05), with the quadratic model showing excellent fit (R² = 0.9924). Optimal conditions were identified as 3% extract concentration, pH 6.0, and 12 days of incubation, under which cordycepin reached 0.995 mg/L, substantially higher than the control (0.693 mg/L). These findings demonstrate that agricultural by-product extracts, particularly onion peel, can serve as effective and economical substrates for enhancing cordycepin biosynthesis while supporting sustainable bioprocessing strategies in C. militaris cultivation.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Mycelial culture preparation
Preparation of liquid culture media
Cultivation of C. militaris
Biomass yield determination
Determination of cordycepin content
Antioxidant activity measurement
Optimization using response surface methodology(RSM)
Statistical analysis
RESULTS AND DISCUSSION
Mycelial biomass yield of C. militaris in different substrates
Quantitative analysis of cordycepin in C. militaris
Assessment of antioxidant properties in C. militaris culture broth
Optimization of culture conditions using response surface methodology
CONCLUSION
ACKNOWLEDGEMENTS
REFERENCES

키워드

저자

• Si Young Ha [ Department of Environmental Materials Science, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Woo Seok Lim [ Department of Environmental Materials Science, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Hyeon Cheol Kim [ Department of Environmental Materials Science, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Jae-Kyung Yang [ Department of Environmental Materials Science, Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635