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An Efficient In Vitro Culture System for Pteris nipponica W.C.Shieh

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    Journal of Forest and Environmental Science KCI 등재 바로가기
  • 통권
    제41권 제4호 (2025.12)바로가기
  • 페이지
    pp.464-469
  • 저자
    Jeong Min Seo, Jae Ik Nam, Chang Ho Ahn
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A479138

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원문정보

초록

영어
Pteris nipponica W.C.Shieh, an evergreen perennial fern with high ornamental value, is an endangered and ecologically important plant in Korea. This study was conducted to optimize the culture conditions for spore germination, sporophyte formation, and subsequent growth, thereby establishing an efficient mass propagation system for P. nipponica. To investigate the effect of ammonium nitrate (NH4NO3) on sporophyte formation and growth, prothalli were cultured for 6 weeks on half-strength MS basal medium containing different concentrations of NH4NO3. The M-3 medium, containing half the standard concentration of NH4NO3, achieved the highest sporophyte formation rate of 58.8%, significantly surpassing M-1 (27.5%) and M-2 (23.8%). Statistical analysis revealed that the concentration of NH4NO3 had a significant effect on sporophyte formation (p=0.0039). Furthermore, the M-3 medium significantly promoted sporophyte growth indicators such as root number, root length, shoot length, and shoot width compared to the M-1 and M-2 media. Therefore, the optimal nitrogen source for effective sporophyte formation and development in P. nipponica was identified as a half concentration of NH4NO3 (5.15 mM) in medium. The formed young sporophytes were transplanted into trays containing sphagnum moss for 4 weeks of acclimatization before being moved to a greenhouse. After 12 weeks, a high survival rate of over 95% was observed. The culture conditions and acclimatization methods established in this study are expected to be practically applicable to the efficient mass production of P. nipponica and various other fern species.

목차

Abstract
Introduction
Materials and Methods
Spore collection, disinfection, and inoculation
Effect of medium components on sporophyteformation and growth
Acclimatization of regenerated sporophytes
Statistical analysis
Results and Discussion
Effect of medium components on sporophyte formation and growth
Acclimatization of regenerated sporophytes
Conclusion
Acknowledgements
References

키워드

ammonium nitrate fern prothallus pteridaceae sporophyte

저자

  • Jeong Min Seo [ Division of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong 30129, Republic of Korea ]
  • Jae Ik Nam [ Division of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong 30129, Republic of Korea ]
  • Chang Ho Ahn [ Division of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong 30129, Republic of Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    Journal of Forest and Environmental Science [산림과학연구]
  • 간기
    계간
  • pISSN
    2288-9744
  • eISSN
    2288-9752
  • 수록기간
    1981~2025
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 526 DDC 634

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