The development of forensic science has significantly contributed to the resolution of criminal cases and the accuracy of legal judgments through the utilization of scientific evidence. In particular, the use of DNA barcoding to identify plant and animal species in forensic science has become an essential and reliable method because it can accurately distinguish species that are difficult to distinguish by morphological characteristics alone. However, in the case of rice species identification, it was confirmed that DNA barcoding, which primarily targets regions with low intra-species variation due to the characteristics of rice breeding development in Korea, has certain limitations. In this study, focused on an embezzlement case involving rice species identification, evaluated the limitations of three conventional DNA barcodes and five rice-specific DNA barcodes in forensic analysis. To address these limitations, we evaluated the applicability of a commercially developed rice species identification kit based on single nucleotide polymorphism (SNP) identification technology. The application of the commercial kit in rice species identification demonstrated high sensitivity and specificity, even with small amounts of test samples. Additionally, it allowed for the determination of rice species in a shorter time frame compared to traditional DNA barcoding methods. As a results, the application of commercial kits in future cases related to rice species identification may help to serve as an reliable forensic biology analysis.
목차
Abstract Ⅰ. 서론 Ⅱ. 재료 및 방법 1. 시료 2. DNA 분리 및 정량 3. DNA 바코드 증폭 4. DNA 바코드 염기서열 분석 5. 벼(쌀) 품종 상용 키트의 적용 Ⅲ. 결과 및 고찰 1. 범용성 DNA 바코드를 이용한 벼 품종 식별 분석 2. 벼 특이적인 DNA 바코드를 이용한 벼 품종식별 분석 3. 벼(쌀) 품종 상용 키트를 이용한 벼 품종식별 분석 Ⅳ. 결론 Ⅴ. 사사 Ⅵ. 참고문헌
법과학 분야는 사회정의 구현에 있어 크나큰 가치가 있음에도 불구하고 우리나라에서는 이 분야에 대한 인식이 미흡하여 선진 외국에 비해 침체되어 있는 실정이다. 이에 우리나라에서도 법과학 분야와 관련 있는 학계, 연구기관, 수사기관 등 유관 단체들로 구성된 한국 법과학회를 창립하여 이 분야를 활성화 시켜 과학수사를 한층 더 발전시키기 위함을 목적으로 한다.