Earticle

초록

영어

CRISPR/Cas9 ribonucleoprotein (RNP)-mediated gene editing has recently been applied to Ganoderma lucidum as a promising tool for functional genomics and strain improvement. However, the multinuclear nature of this basidiomycete can result in genetic mosaicism, raising concerns about the long-term stability of edited strains. In this study, we report the occurrence of revertant phenotypes in CRISPR/Cas9-edited transformants of G. lucidum. Although the edited colonies initially exhibited the expected phenotype, repeated subculturing led to the reappearance of wild-type phenotypes. PCR and sequencing analyses revealed the coexistence of edited and non-edited nuclei, and the progressive loss of edited genotypes over successive generations. These findings demonstrate that multinuclearity is a key factor contributing to the instability of CRISPR/Cas9-based edits in G. lucidum. This brief report provides the first direct documentation of revertant occurrence in edited G. lucidum strains and underscores the need for rigorous selection strategies and novel approaches to secure stable homokaryotic transformants in mushroom genetic engineering.

목차

ABSTRACT
적요
감사의 글
REFERENCES

키워드

저자

• 김민식 [ Minseek Kim | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 오민지 [ Min Ji Oh | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 임지훈 [ Ji-Hoon Im | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 이은지 [ Eun-Ji Lee | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 우성이 [ Sung-I Woo | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 오연이 [ Youn-Lee Oh | 농촌진흥청 국립원예특작과학원 버섯과 ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635