This study aimed to select Perenniporia fraxinea isolates that form mycelial mats with excellent texture and ultimately to use them to produce mycelial based leather (MBL). Among 10 P. fraxinea isolates, JK-M0825 had the fastest mycelial growth rate on malt extract-yeast extract glucose medium. Polymorphic DNA of P. fraxinea isolates was detected using ultra-fast photonic polymerase chain reaction, which showed DNA diversity. The ribosomal DNA-internal transcribed spacer sequence of JK-M0825 was 99% identical to that of P. fraxinea isolates registered in GenBank. Four P. fraxinea isolates formed high-quality mycelial mats on sawdust substrates, and JK-M0825 produced the thickest mycelial mat (3.8 mm). JK-M0825 had the highest tensile strength (6.08 N/mm2) and an elongation rate of 32.93±6.31%. In addition, the mycelia of JK-M0825 formed uniformly on a large-scale culture medium (90 cm x 90 cm) and formed a textured mycelial mat. These results suggest that JK-M0825 is appropriate for producing MBL.
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ABSTRACT 서론 재료 및 방법 아까시재목버섯균주 및 배양 균사체 매트 생산 PCR 다형성분석 균사체 매트의 인장강도 및 연신율 결과 및 고찰 아까시재목버섯균주의 배양적 특성 아까시재목버섯균주의 PCR다형성분석 ITS-rDNA염기서열분석 아까시재목버섯균주의 균사체매트생산 균주간 균사 체매트의 인장강도 및 연신율 적요 감사의 글 REFERENCES