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Original Article
Diazinon causes oxidative stress-mediated damage to neonatal mouse testes
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- 발행기관
- 한국동물생명공학회(구 한국동물번식학회) 바로가기
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- 간행물
- Journal of Animal Reproduction and Biotechnology 바로가기
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- 통권
- Volume. 40 No. 3 (2025.09)바로가기
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- 페이지
- pp.145-153
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A474290
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원문정보
초록
- 영어
- Background: Diazinon (DZN), a widely used organophosphorus pesticide, exerts toxic effects on the reproductive system. In this study, we investigated its effects on neonatal testicular development using mouse testicular fragments cultured in vitro. Methods: Male mouse testis fragments were cultured in vitro with varying concentrations of DZN for 5 days to assess spermatogenic toxicity. Histology, immunostaining, DHE staining, qPCR, and Western blotting were used to evaluate cellular damage, oxidative stress, and gene/protein expression. Results: Histological analysis showed that 50-100 μM diazinon (DZN) induced vacuolization of seminiferous tubules, whereas lower concentrations had no significant morphological effects. Gene and protein expression analyses revealed substantial reductions in both undifferentiated and differentiated germ cell markers (SALL4, DDX4, and SYCP3), indicating germ cell loss. Immunostaining confirmed decreased proportions of DDX4- and SYCP3-positive cells in DZN-treated testes. Additionally, DZN impaired Sertoli and Leydig cell function, as shown by downregulation of key markers (SOX9, WT1, INSL3, and 3β-HSD), verified through immunostaining and western blotting. DZN also elevated reactive oxygen species (ROS) levels, as indicated by intense DHE staining in various testicular cell types. Correspondingly, oxidative stress-related genes (NRF2, HO-1, SOD1, and catalase) and HO-1 protein levels were significantly upregulated, confirming ROS overproduction. Conclusions: Overall, these findings suggest that DZN induces germ cell loss and disrupts the Sertoli and Leydig cell functions in neonatal testes, primarily via oxidative stress and ROS-mediated damage.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Mouse testicular fragment (MTF) culture
Histology and immunostaining
RNA extraction and quantitative real-time polymerase chain reaction
Immunoblotting
Statistical analyses
RESULTS
Effects of DZN on neonatal germ cells
Impacts of DZN on the Sertoli cells and steroidogenic activity of neonatal testes
DZN increases ROS production in neonatal testes
DISCUSSION
CONCLUSION
REFERENCES
저자
간행물 정보
발행기관
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- 발행기관명
- 한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
- 설립연도
- 1976
- 분야
- 농수해양>축산학
- 소개
- 동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.
간행물
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- 간행물명
- Journal of Animal Reproduction and Biotechnology
- 간기
- 계간
- pISSN
- 2671-4639
- eISSN
- 2671-4663
- 수록기간
- 2019~2026
- 십진분류
- KDC 527 DDC 636
이 권호 내 다른 논문 / Journal of Animal Reproduction and Biotechnology Volume. 40 No. 3
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