Earticle

초록

영어

Background: Premature ovarian insufficiency (POI) affects reproductive capacity and endocrine function in women of reproductive age. Preclinical models that reliably recapitulate POI are needed to evaluate ovarian-protective strategies. Methods: Female C57BL/6 mice received a single intraperitoneal injection of cyclophosphamide (120 mg/kg) plus busulfan (30 mg/kg). A subset received an additional cyclophosphamide booster (60 mg/kg) at week 4. Body weight, serum estradiol-2 (E2), and ovarian index (ovary weight compared to control ovary) were assessed weekly for six weeks. Follicle counts were performed on H&E-stained ovarian sections at weeks 1 through 6. Results: Animals experienced a transient ~ 5% body-weight loss in week 1 with full recovery by week 2 (p > 0.05 vs. controls). Ovarian index declined by ~ 50% in the single-injection group by week 4 and remained stable through week 6 (p < 0.05). The booster group exhibited a comparable reduction by week 4 that persisted at weeks 5 and 6 (p > 0.05), indicating maintenance, but no further exacerbation, of ovarian atrophy. Serum E2 dropped by 40% at week 6 in single-injection mice, whereas booster-injected mice showed a 74% decline by week 5 (p < 0.05). Histologically, follicle counts decreased by 84% in the single-injection group (not significant) and by 100% in the booster group by week 6 (p < 0.05). Conclusions: A single dose of cyclophosphamide and busulfan induces sustained ovarian atrophy characteristic of premature ovarian insufficiency (POI), and a delayed booster accelerates hormonal decline without further mass loss. This model offers a standardized platform for testing interventions to preserve ovarian function.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Animals
Ovarian dysfunction mouse model
Blood collection and hormone assays
Histology and hematoxylin-eosin (H&E) staining and follicle counting
Statistical analysis
RESULTS
Induction and characterization of ovarian dysfunction in mice
Dynamics of ovarian follicle
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Jun Mo Kang [ Department of Biomaterials Engineering, School of Medicine and Biomedical Science, College of Life Science, CHA University/CHA Advanced Research Institute, CHA Bundang Medical Center, Seongnam 13488, Korea ]
• Hey Jin Lee [ Department of Biomaterials Engineering, School of Medicine and Biomedical Science, College of Life Science, CHA University/CHA Advanced Research Institute, CHA Bundang Medical Center, Seongnam 13488, Korea ]
• Seung Hwa Baek [ Department of Biomaterials Engineering, School of Medicine and Biomedical Science, College of Life Science, CHA University/CHA Advanced Research Institute, CHA Bundang Medical Center, Seongnam 13488, Korea ]
• Hye-Lim Kang [ Department of Biomaterials Engineering, School of Medicine and Biomedical Science, College of Life Science, CHA University/CHA Advanced Research Institute, CHA Bundang Medical Center, Seongnam 13488, Korea ]
• Sae-Byeok Hwang [ Department of Biomaterials Engineering, School of Medicine and Biomedical Science, College of Life Science, CHA University/CHA Advanced Research Institute, CHA Bundang Medical Center, Seongnam 13488, Korea ]
• Eunju Kang [ Department of Biomaterials Engineering, School of Medicine and Biomedical Science, College of Life Science, CHA University/CHA Advanced Research Institute, CHA Bundang Medical Center/Department of Biochemistry, School of Medicine and Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636