Earticle

초록

영어

Purpose: This study systematically evaluates the antioxidant, tyrosinase-inhibitory, L-DOPA oxidation–suppressive, and anti-inflammatory activities of Crepidiastrum sonchifolium depending on plant part, pretreatment method, and hot-water extraction condition, with the aim of proposing an optimal utilization strategy. Methods: Leaves and roots were pretreated as fresh or dried, sliced (5×5 mm) or ground (500 W, 30 s), and extracted in hot water at 70℃–100℃ for 30–120 min. Total phenolic content (TPC), total flavonoid content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, tyrosinase inhibition, L-DOPA oxidation suppression, human dermal fibroblast neonatal (HDFn) cell viability, and lipopolysaccharide (LPS)–induced tumor necrosis factor-α (TNF-α) suppression were analyzed. Results: Ground dried leaf extract obtained at 90℃ for 120 min exhibited high antioxidant activity with 21.7 mg gallic acid equivalents (GAE)/g dry weight (DW) TPC and a DPPH half-maximal effective concentration (EC50) of 4.23 mg/mL. Root extracts displayed lower antioxidant activity but stronger enzymatic inhibitory activities (tyrosinase inhibition 24.73%; L-DOPA oxidation suppression 14.29%). Cytotoxicity was negligible, maintaining >94% HDFn viability at 100 μg/mL, while TNF-α expression decreased by up to 14.7%. Conclusion: These findings suggest that leaf extracts are suitable for antioxidant and anti-inflammatory applications, whereas root extracts show promise as inhibitors of melanogenesis-related enzymatic steps. Overall, C. sonchifolium exhibits considerable potential as a natural functional resource.

목차

Abstract
Introduction
Methods
1. 시료 전처리 및 추출물 제조
2. 항산화 활성 및 페놀계 화합물 함량 분석
3. Tyrosinase 및 L-DOPA 산화 억제 활성 평가
4. 세포 생존율 평가
5. 항염 효능 평가
6. 통계 분석
Results and Discussion
1. 추출 조건에 따른 생리활성 변화
2. 시료 형태에 따른 C. sonchifolium 추출물의 생리활성 변화
3. C. sonchifolium 추출물의 EC50 기반 항산화 활성 평가
4. C. sonchifolium 추출물의 tyrosinase 저해 및 L-DOPA 산화억제 효과
5. C. sonchifolium 추출물의 독성 평가
6. C. sonchifolium 추출물의 TNF-α 억제 효과
Conclusion
References
국문초록
中文摘要

키워드

저자

• Kwang Hee Kim [ 김광희 | Department of Chemical Engineering, Soongsil University, Seoul, Korea ]
• Tae Eun Kim [ 김태은 | of Bio-Cosmetics Engineering, Division of Convergence, Sungkyul University, Anyang-si, Gyeonggi-do, Korea ]
• Yeo Jin Kim [ 김여진 | Department of Chemical Engineering, Soongsil University, Seoul, Korea ]
• Nam Hee Lee [ 이남희 | Department of Chemical Engineering, Soongsil University, Seoul, Korea ]
• Hee Wook Ryu [ 류희욱 | Department of Chemical Engineering, Soongsil University, Seoul, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  아시안뷰티화장품학술지 [Asian Journal of Beauty and Cosmetology]

• 간기

  계간

• pISSN

  2466-2046

• eISSN

  2466-2054

• 수록기간

  2016~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 593 DDC 646