Earticle

초록

영어

Pseudolysimachion nakaianum (Ohwi) T. Yamaz. is an endemic and important species in the forest ecosystems of South Korea. This study aimed to develop an in vitro propagation system for P. nakaianum to enhance the availability of this rare plant for commercial horticulture and restoration purposes. We utilized different explants like cotyledon (excised after 2 weeks), petiole and leaf (collected after 8 weeks) from in vitro seedlings to assess the impact of five different cytokinins on adventitious shoot organogenesis. In both cotyledon and petiole explants, half-strength Murashige and Skoog (MS) medium, supplemented with 2 mg L-1 thidiazuron (TDZ) and 0.05 mg L-1 1-naphthaleneacetic acid (NAA) evoked the highest rate of shoot induction (61.1%±9.6% and 26.7%±8.8%, respectively) and the highest number of shoots (3±0.6 and 4.4±0.5, respectively) per explant. In contrast, leaf explants showed no in vitro regeneration. Multiple shoots originating from the explants were separated individually and transferred to half-strength MS medium supplemented with 30 g L-1 sucrose and 3 g L-1 GelriteTM. P. nakaianum plantlets were transplanted into plastic pots filled with a mixture of coco peat, decomposed granite, peat, and perlite (5:1:1:3) and approximately 70% the plants were survived for 4 weeks. This protocol outlines an effective method for the direct in vitro regeneration of P. nakaianum from cotyledon and petiole explants providing valuable information for the conservation of this species.

목차

Abstract
Introduction
Materials and Methods
Plant materials and explant sterilization
Effects of cytokinins on shoot induction in from cotyledons
Effects of cytokinins on shoot induction in from petioles and leaves
Aclimatization of regenerated plants
Statistical analysis
Results
Effects of cytokinins on shoot induction from cotyledons
Effects of cytokinins on shoot induction from petioles and leaves
Aclimatization of regenerated plants
Discussion
Conclusions
Acknowledgements
References

키워드

저자

• Jae Ik Nam [ Division of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong 30129, Republic of Korea ]
• Sang Yeol Yoo [ Division of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong 30129, Republic of Korea ]
• Chang Ho Ahn [ Division of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong 30129, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634