Comprehensive Comparison of Phytochemical Contents in Acclimatized In Vitro-Cultured and Naturally Grown Wild Garlic (Allium victorialis var. platyphyllum) Seedlings at Similar Developmental Stages
Mi Jin Jeong, Hak Gon Kim, Seong Hyeon Yong, Do Hyun Kim, Kwan Been Park, Seung A Cha, Ji Hyun Lee, Seon A Kim, Myung Suk Choi
언어
영어(ENG)
URL
https://www.earticle.net/Article/A473942
※ 기관로그인 시 무료 이용이 가능합니다.
4,900원
원문정보
초록
영어
Wild garlic (Allium victorialis var. platyphyllum, AVVP), a valuable wild vegetable and medicinal plant, was studied to compare the chemical compositions of plants propagated by different methods. This study compared the chemical profiles of in vitro plants acclimatized in a greenhouse and ex vivo plants propagated from seeds in a forest. Both groups were derived from the same clonal line and harvested after 12 months of growth. The moisture content of acclimatized in vitro plants and ex vivo propagated plants was similar, and the extraction yield of acclimatized in vitro plants was higher. The inorganic content was generally higher in the shoot than in the bulb, with ex vivo propagated plants showing higher inorganic content in the bulb. The total sugar content was higher in acclimatized in vitro plants in the shoot, and similar in the bulb. The amount of carbohydrates was similar in the shoot, and higher in ex vivo propagated plants in the bulb. The content of moisture, total dietary fiber, and free sugar was high in acclimatized in vitro plants in both shoot and bulb. The content of crude oil, free amino acid, and the total polyphenol was higher in the shoot of ex vivo propagated plants and in the bulb of acclimatized in vitro plants. A total of 36 types of volatile compounds were detected, and dimethyl sulfone was the main component, and the types of compounds were similar in acclimatized in vitro plants and ex vivo propagated plants. Antioxidant activity was higher in ex vivo propagated plants compared to acclimatized in vitro plants. These findings indicate that chemical differences are primarily influenced by environmental conditions rather than plant age or genetics. This study provides valuable insights for the optimized propagation and use of wild garlic and similar medicinal plants in both forest and controlled environments.
목차
Abstract Introduction Materials and Methods Materials of AVVP Extraction yield Inorganic determination Moisture content determination Crude ash determination Total sugar and free sugar determination Carbohydrate determination Insoluble holocellulose fibers determination Total dietary fiber determination Free amino acid determination Total polyphenol determination Volatile compound determination Antioxidant activity Statistical analysis Results Extraction yield Inorganic content Moisture content Crude ash content Total sugar content Free sugar content Carbohydrate content Total polyphenol content Insoluble holocellulose fiber content Total dietary fiber content Free amino acid content Volatile compound analysis Antioxidant activity Comparison of ingredient content in in vitro and ex vivo plants Discussion Conclusion Acknowledgements References
Mi Jin Jeong [ Plant Conservation Division, Korea National Arboretum of the Korea Forest Service, Pocheon 11186, Republic of Korea ]
Hak Gon Kim [ Forest Research Department, Gyeongsangnam-do Forest Environment Research Institute, Jinju 52615, Republic of Korea ]
Seong Hyeon Yong [ Division of Forest Biodiversity, Korea National Arboretum, Pocheon 11186, Republic of Korea ]
Do Hyun Kim [ Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science), Jinju 52828, Republic of Korea ]
Kwan Been Park [ Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science), Jinju 52828, Republic of Korea ]
Seung A Cha [ Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science), Jinju 52828, Republic of Korea ]
Ji Hyun Lee [ Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science), Jinju 52828, Republic of Korea ]
Seon A Kim [ Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science), Jinju 52828, Republic of Korea ]
Myung Suk Choi [ Division of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science), Jinju 52828, Republic of Korea ]
Corresponding Author