Earticle

초록

영어

Background: Heat stress during summer impairs reproductive performance in sows, causing summer infertility. Cytochrome P450 1A2 (CYP1A2) plays a crucial role in steroid hormone metabolism in the liver and has been shown to be upregulated under stress conditions. However, the effect of seasonal heat stress on CYP1A2 expression in sow ovaries remains poorly understood. Methods: Ovaries were collected from crossbred sows during different seasons in Korea. The control group (CON) was exposed to optimal temperatures for swine rearing (22.0-24.5℃) during spring, while the heat-stressed group (HS) experienced high temperatures (30.5-37.0℃) during summer. Temperature-humidity index (THI) was calculated to assess thermal stress severity. CYP1A2 expression was analyzed using Western blotting and immunohistochemistry (IHC), and these values were statistically compared with THI by Pearson’s correlation analysis. Results: The HS exhibited significantly higher THI values compared to the CON (88.11 vs. 66.67). Western blot analysis revealed significant upregulation of CYP1A2 expression in the HS compared to CON (1.89 vs. 1.00). IHC demonstrated that CYP1A2 was specifically localized in granulosa cells of mature follicles, with a significantly higher proportion of CYP1A2-positive follicles in the HS (15.92%) compared to CON (9.08%). When the obtained values were compared with THI values, CYP1A2 expression showed a strong positive correlation with THI (r = 0.41 and 0.74). Conclusions: This study revealed that heat stress can alter the ovarian microenvironment, including the expression of CYP1A2. These findings emphasize the need for effective thermal management strategies to mitigate heat stress-induced reproductive dysfunction in livestock production.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Ethics statement
Animals and sampling
Temperature-humidity index (THI) calculation and stress thresholds
Western blotting for quantitative expression of CYP1A2
Immunohistochemistry (IHC) for detection CYP1A2 in ovarian tissue
Statistical analysis
RESULTS
THI values and heat stress severity in different seasons
CYP1A2 expression in the ovaries of sows in different seasons
Immunohistochemistry of CYP1A2 in the ovaries of sows in different seasons
DISCUSSION
CONCLUSION
SUPPLEMENTARY MATERIALS
REFERENCES

키워드

저자

• Tae-Gyun Kim [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Hyun-Seung Lee [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Sung-Ho Kim [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Young-Bum Son [ College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea ]
• Seong-Eun Heo [ Gyeongsangbuk-Do Livestock Research Institute, Yeongju 36052, Korea ]
• Do-Yoon Kim [ Gyeongsangbuk-Do Livestock Research Institute, Yeongju 36052, Korea ]
• Seung-Joon Kim [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Won-Jae Lee [ College of Veterinary Medicine, Kyungpook National University/Institute for Veterinary Biomedical Science, Kyungpook Natitonal University, Daegu 41566, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636