Frequency-specific neuromuscular adaptations: comparative effects of high- and low-frequency neuromuscular electrical stimulation on muscle function and quality
This study investigated the frequency-specific effects of high- and low- frequency neuromuscular electrical stimulation (NMES) on neuromus-cular function and muscle quality. Sixteen healthy young males were randomly assigned to either a high-frequency stimulation group (HFES, 100 Hz; n=8) or a low-frequency stimulation group (LFES, 50 Hz; n=8) for 6 weeks of NMES intervention. Neuromuscular function was as-sessed using maximum voluntary isometric contraction (MVIC), while muscle quality of the rectus femoris (RF) and vastus lateralis (VL) was evaluated via ultrasound echo intensity (EI). Assessments were taken at baseline, midintervention (week 3), and postintervention (weeks 6, 8 and 10). HFES significantly increased MVIC during the intervention, indi-cating an immediate neuromuscular improvement. In contrast, LFES showed delayed effects, with significant EI improvements in RF and VL emerging only after the intervention ended. Statistical analysis revealed a significant interaction effect between time and intervention for MVIC and EI, highlighting the frequency-dependent nature of NMES adapta-tions. These results suggest that NMES induces neuromuscular adap-tations in healthy adult males, with HFES promoting immediate gains and LFES leading to delayed benefits. Thus, frequency selection is criti-cal in designing effective NMES protocols.
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Abstract INTRODUCTION MATERIALS AND METHODS Participants Experimental protocol NMES intervention Electromyography Force Ultrasound images Statistical analysis RESULTS Force Muscle activation Muscle structure and quality DISCUSSION CONFLICT OF INTEREST ACKNOWLEDGMENTS REFERENCES
Hyeon-Deok Jo [ Sports Science Research Institute, Kyungpook National University, Daegu, Korea ]
Maeng-Kyu Kim [ Sports Science Research Institute, Kyungpook National University/Sports Medicine Lab, Department of Physical Education, Kyungpook National University, Daegu, Korea ]
Corresponding Author