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Adaptation of the Hypoosmotic Swelling Test to Evaluate Membrane Integrity of Boar Spermatozoa

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 31 No 2 (2007.06)바로가기
  • 페이지
    pp.121-126
  • 저자
    Hyun-Yong Jang, Hee-Tae Cheong, Hwan-Sub Heang, Jong-Taek Kim, Choon-Keun Park, Hak-Kyu Lee, Boo-Keun Yang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A46742

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초록

영어
The objective of this study was to establish the optimal conditions for hypoosmotic swelling (HOS) test to assess the functional integrity of the membranes of boar fresh or frozen/thawed spermatozoa. When pooled semen sample was incubated for 30 min at 37℃ with different test solution of varied osmolarity, the highest percentage of HOS positive spermatozoa was observed in a 150 mOsmol fructose/Na-citrate solution (33.6%). Incubation time did not affect significantly the score of HOS positive spermatozoa observed in a 150 mOsmol fructose/Na-citrate solution at 37℃, but the osmolarity affected the score of HOS positive spermatozoa under the same condition above. Fresh semen was significantly better than frozen/thawed semen in semen parameters evaluated such as motility, viability, membrane integrity and lipid peroxidation (p<0.05). In the relationships of sperm parameters, motility vs viability, motility vs membrane integrity and viability vs membrane integrity were positively correlated (0.82~0.94) but lipid peroxidation vs other estimated factors was negatively correlated (-0.90~-0.98). Among the evaluation methods, motility vs viability, motility vs membrane integrity and lipid peroxidation vs other estimated factors were significantly correlated (p<0.05). These results of this study indicate that the optimal condition of HOST in boar spermatozoa is a 150 mOsmol fructose/Na-citrate solution for 30 min incubation at 37℃ and HOST can substitute the examination of motility, viability and lipid peroxidation.

목차

ABSTRACT
 INTRIDUSTION
 MATERIALS AND METHODS
  Semen Preparation
  Hypoosmotic Swelling Test(HOST)
  Comparison of Hypoosmotic Solutions
  Comparison of Incubation Time
  Comparison on Osmolarity
  Comparison of semen Status
  Quantification of Lipid Peroxidation
  Statistical Analysis
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Hypoosmotic swelling test(HOST) Boar spermatozoa Spermatozoal plasma membrane Frozen/thawed semen Lipid peroxidation

저자

  • Hyun-Yong Jang [ College of Animal Life Science ]
  • Hee-Tae Cheong [ School of Veterinary Medicine, Kangwon National University ]
  • Hwan-Sub Heang [ Gangwon Livestock Research Center ]
  • Jong-Taek Kim [ Gangwon Livestock Research Center ]
  • Choon-Keun Park [ College of Animal Life Science ]
  • Hak-Kyu Lee [ GRRC, Hankyong National University ]
  • Boo-Keun Yang [ College of Animal Life Science ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 31 No 2

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