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Effects of Mono-and Polysaccharides on In Vitro Fertility of Boar Spermatozoa

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 31 No 2 (2007.06)바로가기
  • 페이지
    pp.115-120
  • 저자
    In-Sun Hwang, Hee-Tae Cheong, Boo-Keun Yang, Choung-Ik Kim, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A46741

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원문정보

초록

영어
This study was conducted to examine the effect of several saccharides on the induction of capacitation and acrosome reaction (AR) and to examine the effects of mono and polysaccharides on the penetration activity of boar spermatozoa. Spermatozoa were inseminated in medium with fucose, galactose and mannose as monosaccharide, and fucoidan, galactan and mannan as polysaccharide. The penetration rates were significantly (p<0.05) lower in medium with galactose (40.6%), mannose (38.1%), fucose (41.6%) and fucoidan (36.6%) compared with control (56.7%). The rates of AR were increased (40.7 to 59.8%) by the preincubation periods prolonged from 0 to 4 hr (p<0.05). Similar tendencies were observed in AR when spermatozoa were treated with monosaccharides, but not significantly differ among the groups treated with different time of preincubation with some exception of galactose. When spermatozoa were treated with polysaccharides, the rates of AR were significantly (p<0.05) increased by preincubation time prolonged from 0 to 4 hr with an exception of fucoidan. In conclusion, the present study suggests that penetration rate of spermatozoa is higher in presence of polysaccharides than monosaccharides. Also, it may resume that the comparing to control, the all saccharides (L-fucose, D-galactose, D-mannose, fucoidan, galactan and mannan)-treated groups slightly increase the AR pattern as preincubation time prolonged.

목차

ABSTRACT
 INTRIDUSTION
 MATERIALS AND METHODS
  Oocytes Preparation
  Treatment of Saccharides and In Vitro Fertilization
  Chlortetracycline(CTC) Assessment of Spermatozoa
  Evlauation of Sperm Penetration
  Statistical Analysis
 RESULTS
 DISCUSSION
 REFERENCES

키워드

Acrosome reaction In vitro fertility Mono/polysaccharides Pigs

저자

  • In-Sun Hwang [ College of Animal Life Science ]
  • Hee-Tae Cheong [ School of Veterinary Medicine, Kangwon National University ]
  • Boo-Keun Yang [ College of Animal Life Science ]
  • Choung-Ik Kim [ College of Animal Life Science ]
  • Choon-Keun Park [ College of Animal Life Science ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 31 No 2

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