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Analysis of TIMP-2 and Vimentin Protein Expression and Epigenetic Reprogramming in Cloned Bovine Palcentae

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 31 No 2 (2007.06)바로가기
  • 페이지
    pp.97-102
  • 저자
    Hong-Rye Kim, Rong-Xun Han, Hye-Ran Lee, Jong-Taek Yoon, Hee-Tae Cheong, Chang-Sik Park, Dong-Il Jin
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A46736

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원문정보

초록

영어
The objective of this study was to analyzed pattern of proteins expression abnormally in cloned bovine placenta. TIMP-2 protein whose function is related to extracellular matrix degradation and tissue remodeling processes was one of differentially up-regulated proteins in SCNT placenta. And one of down-regulated protein in SCNT placenta was identified as vimentin protein that is presumed to stabilize the architecture of the cytoplasm. The expression patterns of these proteins were validated by Western blotting. To evaluate how regulatory loci of TIMP-2 and vimentin genes was programmed reprogramming in cloned placenta, the status of DNA methylation in the promoter region of TIMP-2 and vimentin genes was analyzed by sodium Bisulfite mapping. The DNA methylation results showed that there was not difference in methylation pattern of TIMP-2 and vimentin loci between cloned and normal placenta. Histone H3 acetylation state of the nucleosome was analyzed in the cloned placental and normal placenta by Western blotting. A small portion of the protein lysates were subjected to Western blotting with the antibodies against anti acetyl-Histone H3. Overall histone H3 acetylation state of SCNT placenta was significantly higher than those of normal placenta cells. It is postulated that cloned placenta at the end of gestation seems to be unusual in function and morphology of placenta via improper expression of TIMP-2 and vimentin by abnormal acetylation states of cloned genome.

목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Placental Samples
  Extraction of Solubilized Proteins from Bovine Placentae
  2-D Gel Electrophoresis, MALDI-TOF MS and Protein Identification
  Genomic DNA Isolation and Bisulfite Treatment
  PCR Amplification and Sequencing of the Bisulfite-Rreated Genomic DNA
  Western Blot Analysis
  Statistical Analysis
 RESULTS
  TIMPS-2 Expression in SCNT Plancenate
  Vimentin in SCNT Placentae
  Overall Histone H3 Acetylation State of SCNT Placenta
 DISCUSSION
 REFERENCES

키워드

Bovine cloned placenta TIMP-2 Vimentin Methylation Acetylation

저자

  • Hong-Rye Kim [ Research Center for Transgenic Cloned Pigs, Chungnam National University ]
  • Rong-Xun Han [ Research Center for Transgenic Cloned Pigs, Chungnam National University ]
  • Hye-Ran Lee [ Research Center for Transgenic Cloned Pigs, Chungnam National University ]
  • Jong-Taek Yoon [ Genetic Engineering Institute, Hankyong National University ]
  • Hee-Tae Cheong [ School of Veterinary Medicine, Kangwon National University ]
  • Chang-Sik Park [ Research Center for Transgenic Cloned Pigs, Chungnam National University ]
  • Dong-Il Jin [ Research Center for Transgenic Cloned Pigs, Chungnam National University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 31 No 2

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