Earticle

초록

영어

In vitro development of porcine embryo is affected by culture condition. One possible factor is osmolarity of culture medium. This study examined whether high osmolarity of culture medium at the early culture stage improves development of preimplantation porcine in vitro fertilization (IVF) and nuclear transfer (NT) embryos. NT and IVF embryos were divided into three groups and the basic medium was PZM-3 (250～270 mOsmol, control group). The control group of embryos was cultured in PZM-3 for whole culture period. Other two groups of embryos were cultured in a modified PZM-3 with 0.05 M sorbitol or 0.05 M sucrose (300～320 mOsmol, sorbitol or sucrose group) for the first 2 days, and then cultured in PZM-3 for further culture. NT embryos cultured in sucrose group showed a significantly higher developmental rate to the blastocyst stage with a decreased apoptosis rate compared to the sorbitol (p<0.05). For IVF, sucrose group showed a significantly increased the blastocyst formation rate with a decreased apoptosis rate compared to the control (p<0.05). This study represents that the high osmolarity in the early embryo culture stage can enhance the in vitro development of porcine NT and IVF embryos to the blastocyst stage with reduced apoptosis of cells.

목차

ABSTRACT
 INTRODUCTION
 MATRRIALS AND METHODS
  Collection of Oocytes and IVM
  IVF
  Preparation of Porcine Fetal Fibroblast Cells
  Production of NT Embryos
  In Vitro Culture of Embryos(IVC)
  Apoptosis Assays
  Statistical Analysis
 RESULTS
  Effects of Sorbitol and Surcrose on Development of IVF Embryos
 DISCUSSIONS
 REFERENCES

키워드

저자

• In-Sun Hwang [ National Livestock Research Institute, RDA ]
• Mi-Rung Park [ National Livestock Research Institute, RDA ]
• Hyo-Jin Moon [ National Livestock Research Institute, RDA ]
• Joo-Hyun Shim [ National Livestock Research Institute, RDA ]
• Dong-Hoon Kim [ National Livestock Research Institute, RDA ]
• Byoung-Chul Yang [ National Livestock Research Institute, RDA ]
• Yeoung-Gyu Ko [ National Livestock Research Institute, RDA ]
• Hee-Tae Cheong [ School of Veterinary Medicine, Kangwon National University ]
• Soh-Suk Yang [ National Livestock Research Institute, RDA ]
• Gi-Sun Im [ National Livestock Research Institute, RDA ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636