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Effects of BSA, PVA, Gonadotropins and Follicle Shell on In Vitro Maturation and In Vitro Fertilization of Porcine Oocytes

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 31 No 2 (2007.06)바로가기
  • 페이지
    pp.61-69
  • 저자
    Pei-Qing Cong, Eun-Sook Song, Eui-Sook Kim, Zhao-Hua Li, Yong-Hua Zhang, Young-Joo Yi, Chang-Sik Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A46727

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초록

영어
This study was designed to evaluate effects of BSA, PVA, gonadotropins and follicle shell during IVM of porcine oocytes and subsequent development to the blastocyst stage after IVF. Cumulus oocyte complexes (COCs) were cultured in TCM-199 media containing 4 mg/ml BSA and 1 mg/ml PVA during IVM for 44 hr. To compare the effect of gonadotropins on oocyte maturation, COCs were cultured with FSH+LH, FSH, LH and FSH-LH-free media during IVM, respectively. Also, different number of follicle shells (0, 2, 4 and 6) was used to examine whether the presence of follicle shell in culture medium affects oocyte maturation. The percentages of fertilization and blastocyst formation, respectively, were higher in the medium containing the PVA (49.0 and 17.9%) than those containing the BSA (40.0 and 12.2%). Significantly higher rates of MII oocytes were in the presence of FSH+LH and FSH (88.6 and 85.1%) compared to other treatments (64.0 and 53.4% at LH and FSH-LH-free media). Co-culture with inverted follicle shells in 2 ml maturation medium enhanced the developmental competence of porcine oocytes. In conclusion, PVA could be used as a macromolecules instead of BSA, and FSH and follicle shell played important roles in maturation of porcine oocytes.

목차

ABSTRACT
 INTRIDUSTION
 MATERIALS AND METHODS
  Oocyte Collection and In Vitro maturation(IVM)
  Semen Collection and Liquid Semen Processing
  In Vitro Fertilization(IVF) and Culture of Oocytes
  Evaluation of Oocytes and Embryos Produced In Vitro
  Experimental Designs
  Statistical Analysis
 RESULTS
  Effects of Different mTCM-199 Media on IVM
  Effects of BSA and PVA and IVM
  Effects of BSA and PVA on IVF and Subsequent Development
  Effects of Gonadotropins on IVM
  Effects of Follicle Shell Co-culture on IVM, and Embryos Development
 DISCUSSION
 REFERENCES

저자

  • Pei-Qing Cong [ Research Center for Transgenic Cloned Pigs, Chungnam National University ]
  • Eun-Sook Song [ Research Center for Transgenic Cloned Pigs, Chungnam National University ]
  • Eui-Sook Kim [ Research Center for Transgenic Cloned Pigs, Chungnam National University ]
  • Zhao-Hua Li [ Research Center for Transgenic Cloned Pigs, Chungnam National University ]
  • Yong-Hua Zhang [ Research Center for Transgenic Cloned Pigs, Chungnam National University ]
  • Young-Joo Yi [ Research Center for Transgenic Cloned Pigs, Chungnam National University ]
  • Chang-Sik Park [ Research Center for Transgenic Cloned Pigs, Chungnam National University ] Corresponding author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 31 No 2

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