2024 International Symposium of Institute of Forest Science (2024.10)바로가기
페이지
pp.115-115
저자
Sangjun Lee, Hyunjun Ko
언어
영어(ENG)
URL
https://www.earticle.net/Article/A467147
원문정보
초록
영어
Fallen leaves are often viewed as a nuisance unused forest biomass, contributing to wildfire risks and sewer blockages. However, they also present a valuable resource for producing high-value-added products. In a previous study, we demonstrated the economic feasibility of cultivating Pseudomonas species microorganisms using a hardwood fallen leaf extract (HLE)-based medium. This study focuses on further utilization of the HLE medium to produce levan, a functional biopolymer, by addition of sucrose as a carbon source and cultivation of Pseudomonas chlororaphis. Over a 72-hour cultivation, the cell growth-dependent levan production was monitored, resulting in a yield of 7.1 g/L from 19.6 g/L of sucrose (36.7% conversion yield). The crude levan was purified through a straightforward filtration process. However, the purified levan exhibited a dark-brown color due to the inherent pigmentation of the HLE, which could limit its industrial applications. To address this, we conducted decolorization experiments using eight different materials, with activated charcoal designed for water treatment showing the highest efficacy. Under the optimal decolorization conditions, the produced levan achieved a clear, colorless appearance. Taken together, this study confirms the industrial feasibility of using fallen leaf-based media for the production of high-value bioproducts like levan.