Earticle

초록

영어

The purpose of this study was to investigate the effect of combining low-intensity treadmill exercise with naringenin treatment on the ex-pression of axonal regrowth-related proteins following sciatic nerve in-jury (SNI). The extracts were evaluated for cytotoxicity and cell viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the effects of the extracts were analyzed in vitro using pri-mary cultured Schwann cells and dorsal root ganglion neurons (DRGs). In vivo, axonal regrowth-related protein expression levels and neurite outgrowth were assessed through Western blot and immunofluores-cence staining, respectively. The results indicated that neither extract exhibited cytotoxicity. In primary cultured Schwann cells, 10 μM nar-ingin and 10 μM/50 μM naringenin significantly increased growth asso-ciated protein-43 (GAP-43) expression, while in DRGs, both naringin and naringenin treatments resulted in increased neurite length. For in vivo experiment, all animals were divided into the vehicle group, the nar-ingin-treated group post-SNI (Gin), the naringenin-treated group post-SNI (Genin), the naringin and exercise group post-SNI (Gin+Ex), and the naringenin and exercise group post-SNI (Genin+Ex). Naringenin treat-ment after early SNI enhanced GAP-43 expression. Following 14 days of exercise combined with treatment, both GAP-43 and phosphorylated extracellular signal-regulated kinase levels were significantly increased in the Genin and the Genin+Ex groups, whereas phosphorylated-protein kinase B significantly increased only in the Genin+Ex group. Our findings suggest that naringenin, when used in conjunction with low-intensity treadmill exercise, may effectively promote the expression of axonal growth-related proteins following SNI.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Experimental animals
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay
SNI and extract administration
Low-intensity treadmill exercise
Dorsal root ganglion neuron and Schwann cell culture
Western blot analysis
Immunofluorescence staining
Statistical analysis
RESULTS
Naringin and naringenin prevent the loss of cell viabilityin vitro
Naringenin is more effective in activating regenerative responses in vitro
Naringenin upregulates GAP-43 level in vivo during the early stage of the regeneration
Combined exercise with naringenin activates in vivo protein expression for regeneration
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Chan-Gyun Oh [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ]
• Yoon-A Jeon [ Veterinary Medical Research Institute, Jeju National University, Jeju, Korea ]
• Young Jae Lee [ Veterinary Medical Research Institute, Jeju National University, Jeju, Korea ]
• Tae-Beom Seo [ Department of Kinesiology, College of Natural Science, Jeju National University, Jeju, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613