Earticle

초록

영어

The effects of maternal exercise on hippocampal neurogenesis, syn-aptic protein expression, and gut microbiome composition in the off-spring of older females were investigated. Male offspring from female C57BL/6 mice were divided into four groups: offspring of young female group (CON), offspring of exercised young female group, offspring of advanced-age female group (AMA), and offspring of exercised ad-vanced-age female group (AMA+EX). The exercised group received 8 weeks of treadmill training before and during pregnancy. Male off-spring were assessed at 4 weeks of age. Hippocampal neurogenesis was assessed by 5-bromo-2’-deoxyuridine/neuronal double immunoflu-orescence staining. Expression of synaptic plasticity-related proteins, including brain-derived neurotrophic factor (BDNF) and postsynaptic density protein 95 (PSD-95), was analyzed by Western blot. Gut microbi-ome composition and diversity were assessed using 16S rRNA se-quencing of fecal samples. Offspring born to AMA females had signifi-cantly reduced hippocampal neurogenesis and lower expression levels of BDNF and PSD-95 compared to the CON group. In the AMA+EX group, maternal treadmill exercise significantly improved these deficits, restoring both neurogenesis and synaptic protein expression. In con-trast, gut microbiota analysis showed that microbial richness and alpha diversity were reduced in the offspring of exercised females, despite the relatively high diversity in the CON and AMA groups, especially in the AMA+EX group. Older mothers impair hippocampal neurogenesis and synaptic protein expression in offspring, and alter gut microbial diversi-ty. Maternal exercise may alleviate age-related neurodevelopmental disorders, but may also reduce microbial diversity in the offspring’s gut.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Experiment animals
Exercise protocol
Immunofluorescence
Western blotting for BDNF and PSD-95
Collection of fecal samples and sequencing/metagenomic analysis
RESULTS
Data analysis
The effects of maternal exercise with AMA on neurogenesis in the hippocampus
The effects of maternal exercise with AMA on BDNF and PSD-95 expression in the hippocampus
The effects of maternal exercise with AMA on gut microbiomes
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Sang-Seo Park [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Tae-Woon Kim [ Department of Human Health Care, Gyeongsang National University, Jinju, Korea ]
• Bo-Kyun Kim [ Department of Emergency Technology, College of Health Science, Gachon University, Incheon, Korea ]
• Sang-Hoon Kim [ Department of Neurosurgery, Rutgers Robert Wood Johnson Medical School, The Stat University of New Jersey, Piscataway, NJ, USA ]
• Hye-Sang Park [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613