Earticle

초록

영어

Background: Embryo implantation is a complex process regulated by interactions between endometrial epithelial and stromal cells. The endometrium plays a critical role in this process, providing a supportive environment for embryo attachment. However, conventional 2D cell culture models fail to fully replicate the complex 3D structure and cellular interactions of the endometrium. To overcome these limitations, 3D organoid models have been developed to better mimic the in vivo endometrial environment. Methods: In this study, a multicellular uterine organoid model was developed using porcine endometrial epithelial cells (pEECs) and porcine endometrial stromal cells (pESCs) to evaluate the effects of the endometrial environment on embryo implantation. First, single-cell endometrial organoids (pEOs) were formed by culturing pEECs in Matrigel, and their basic cellular characteristics were assessed. Then, a multicellular uterine organoid model was established by combining pEOs with pESCs. Finally, porcine embryos were co-cultured with this model to examine its effect on embryo attachment. Results: The multicellular uterine organoid model facilitated embryo attachment, demonstrating that the 3D structure and cellular interactions of the endometrium play a significant role in embryo implantation. The presence of both epithelial and stromal cells contributed to a more physiologically relevant environment that supported embryo adhesion. Conclusions: This study demonstrates that a multicellular uterine organoid model can serve as a useful in vitro system for porcine embryo implantation research. This model may contribute to a better understanding of embryo development and implantation mechanisms, with potential applications in regenerative medicine and biotechnology.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Ethical statement
Chemicals
Preparation of endometrial cell line
Establishment of monocellular endometrial organoids(mono-EOs)
Immunofluorescence
Establishment of multicellular endometrial organoids(multi-EOs)
Preparation of porcine embryos and embryo outgrowth with EOs
Statistical analysis
RESULTS
Characterization of pEEOs
Cultivation and characterization of porcine endometrial stromal cells
Embryo outgrowth with multi-pEOs
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Seonggyu Bang [ College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University/College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea ]
• Ayeong Han [ College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University/College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea ]
• Heejae Kang [ College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University/College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea ]
• Ghangyoung Kim [ College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea ]
• Seungjun Lee [ College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea ]
• Yunju Ha [ College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea ]
• Sanghoon Lee [ College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea ]
• Jongki Cho [ College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636