Earticle

초록

영어

Background: The poultry industry experiences genetic losses due to recurring infectious diseases, necessitating effective preservation strategies. Nitric oxide plays a crucial role in male reproduction, and optimal NO (nitric oxide) levels may enhance sperm viability. This study investigated the effects of SNAP (S-nitroso-Nacetylpenicillamine) on the longevity of rooster sperm. Methods: Semen was diluted with Beltsville Poultry Semen Extender-I containing 0 or 25 μM SNAP and stored at 10°C. Sperm motility and acrosome integrity were assessed at 1, 3, and 7 days. NO levels were quantified by DAF-FM diacetate and AI trials were evaluated by fertility and hatchability. Results: On day 1, sperm motility in the SNAP 25 μM-treated group was significantly higher than in the control. NO quantification confirmed that SNAP-treated semen exhibited higher NO levels. For fertilization and hatchability assessment, hens were divided into two groups based on the presumed duration sperm resided in sperm storage tubules. Before artificial insemination, the sperm was preserved at low temperature (10°C) to maintain viability. Fertilization rates were significantly higher in the SNAP-treated group in both short-term and long-term SST storage conditions. However, hatchability was only significantly improved in the SNAP-treated group when fertilization occurred after extended storage. Conclusions: These findings suggest that NO enhances sperm viability and fertility in poultry semen stored at low temperatures. SNAP 25 μM enhances AI efficiency by maintaining sperm viability and extending fertilization potential. Further research is needed to refine NO-based fertility enhancement strategies for avian species.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Experimental animals and semen collection
Reagents and semen extender composition
Semen refrigeration and storage
Sperm morphology and longevity analysis
Acrosome length measurement
Quantification of nitric oxide using DAF-FM diacetate
Fertilization and hatchability assessment following artificial insemination
Statistical analysis
RESULTS
Sperm motility and longevity analysis following SNAP treatment
Acrosome analysis of rooster sperm following SNAP supplementation and low-temperature storage
Quantification of nitric oxide (NO) using DAF-FM diacetate
Effect of low-temperature stored semen on fertilization and hatchability following artificial insemination
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Ga Yeong Lee [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Hamyang 50000, Korea/Department of Animal Resources Science, Kongju National University, Yesan 32439, Korea ]
• Jae-Yeong Lee [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Hamyang 50000, Korea ]
• Yeoung-Gyu Ko [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Hamyang 50000, Korea ]
• Daehyeok Jin [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Hamyang 50000, Korea ]
• Se Young Lee [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Hamyang 50000, Korea ]
• Bongki Kim [ Department of Animal Resources Science, Kongju National University, Yesan 32439, Korea ] Corresponding Author
• Sung Woo Kim [ Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang 25340, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636