Earticle

초록

영어

Background: mTeSR1 is a fully-defined, serum-free medium for the derivation and maintenance of Human embryonic stem cells (ESCs). This study investigates the impact of incorporating mTeSR1 supplement during in vitro culture (IVC) on blastocyst productivity, qualitative characteristics, and outgrowth potential of bovine blastocysts. Methods: In vitro fertilized (IVF) eggs were cultured in IVC medium (control) with the addition of mTeSR1 supplement at concentrations of 1%, 2%, and 5%, respectively. The development rates of fertilized eggs and gene expression patterns of blastocysts were assessed on day 9 of culture. For outgrowth culture, blastocysts were cultured on a mouse embryonic fibroblast feeder cells (MEFs) for 7 days. Results: In vitro development of bovine preimplantation embryos in the 2% mTeSR1 group was significantly higher than in the control (p < 0.05). The apoptotic index in the 2% mTeSR1 group was significantly lower compared to the control (p < 0.05). RTqPCR indicated that SRY-Box Transcription Factor 2 (Sox2) gene expression in the 5% mTeSR1 group was significantly higher than in the control (p < 0.05). The 5% mTeSR1 group also showed significantly higher BCL2 associated X (Bax) expression compared to the control and other mTeSR1 groups. On day 9 pi, blastocysts from the control and 2% mTeSR1 groups were cultured for 7 days. The 2% mTeSR1 group showed higher efficiency in forming dome-shaped colonies with stronger SOX2 expression compared to the control. Conclusions: The mTeSR1 supplement supports preimplantation embryo development and prevents apoptosis in blastocysts, leading to the efficient formation of domeshaped inner cell mass (ICM) colonies.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
In vitro embryo production
Apoptotic cell number in blastocysts and immunostaining
Quantitative PCR of blastocysts
Statistical analysis
RESULTS
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Dohyun Kim [ Department of Animal Biotechnology, Chonbuk National University, Jeonju 54896, Korea ]
• HakKyo Lee [ Department of Animal Biotechnology, Chonbuk National University, International Agricultural Development and Cooperation Center, Chonbuk National University, Jeonju 54896, Korea ]
• Dae-Jin Kwon [ Department of Animal Biotechnology, Chonbuk National University, Jeonju 54896, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2025

• 십진분류

  KDC 527 DDC 636