Earticle

초록

영어

Background: Cisplatin, a chemotherapeutic agent often causes nephrotoxic side effects. Lipopolysaccharide (LPS) is known to induce pro-inflammatory responses, often leading to septic renal injury. We hypothesized that the combination of cisplatin and LPS would amplify renal injury, thereby improving a renal injury model. Therefore, we administered both agents to mice and evaluated renal injury indicators. Methods: Eight-week-old male C57BL/6 mice were injected with cisplatin (8, 10, or 12 mg/kg) and LPS (5 mg/kg) on days 1 and 4 following of each week. Mice were euthanized at specific time points to assess renal injury. Body weight, renal weight, area, and BUN levels were measured to evaluate renal damage. Additionally, hematoxylin and eosin (H&E) and Masson’s trichrome (MT) staining were performed to assess histological changes. Results: The combination of cisplatin and LPS significantly reduced body and renal weight compared to cisplatin alone. A high dose of cisplatin (12 mg/kg) resulted in a 50% mortality, while, lower doses (8 and 10 mg/kg) showed 100% survival. Significant renal injury was observed in the 10 mg/kg cisplatin group administered for two weeks. In the 8 mg/kg cisplatin group, no changes were observed after two weeks, but renal damage appeared after four weeks. Histological evaluations in the 10 mg/kg cisplatin group administered for two weeks showed renal injury features, including tubular damage and fibrosis. Conclusions: Administering cisplatin (10 mg/kg) with LPS for two weeks or cisplatin (8 mg/kg) with LPS for four weeks resulted in a distinct renal injury, effectively establishing a renal injury mouse model.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Chemical induced-renal injury model
Immunohistochemistry
Assessments of BUN
Statistical analysis
RESULTS
Effect of cisplatin and LPS co-administration to generate renal injury model
Dose and duration-dependent effects of cisplatin and LPS co-administration
Histological abnormality and fibrosis in renal injury mouse model
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Sae-Byeok Hwang [ Cell Therapy 3 Center, CHA Advanced Research Institute, CHA Bundang Medical Center, Department of Biomaterials Engineering, School of Medicine and Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea ]
• Soon-Suk Kang [ Cell Therapy 3 Center, CHA Advanced Research Institute, CHA Bundang Medical Center, Seongnam 13488, Korea ]
• Yeonmi Lee [ Cell Therapy 3 Center, CHA Advanced Research Institute, CHA Bundang Medical Center, Department of Biochemistry, School of Medicine and Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea ]
• Eunju Kang [ Cell Therapy 3 Center, CHA Advanced Research Institute, CHA Bundang Medical Center, Department of Biochemistry, School of Medicine and Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636