Earticle

초록

영어

We developed a shoot multiplication protocol for Pseudolysimachion longifolium (L.) Opiz using in vitro cultured seedlings derived from in vitro germinated seeds. To determine the effects of different plant growth regulators (PGRs) on shoot induction and the number of shoots, P. longifolium leaves, petioles, and stems were cultured on half-strength Murashige and Skoog (MS) medium supplemented with 3% sucrose, 0.3% GelriteTM, 0.05 mg L-1 1-naphthaleneacetic acid, and five different types of cytokinins (6-benzylaminopurine, kinetin, thidiazuron (TDZ), zeatin, and 2-isopentenyladenine at 2 mg L-1). Among the five cytokinins tested, stem explants cultured on medium containing TDZ exhibited the best shoot induction (100%) and the highest number of shoots per explant (1.83±0.20). Shoot induction from petiole explants was highest when they were cultured on medium containing zeatin or TDZ. However, no shoot induction was observed from stem or petiole explants when cultured on medium containing kinetin, nor from leaf explants for any of the cytokinin treatments. In vitro generated P. longifolium plantlets were acclimatized in a greenhouse and showed normal growth and development with 90% survival. In conclusion, a regeneration protocol was developed that can be applied for the mass propagation and conservation of P. longifolium.

목차

Abstract
Introduction
Materials and Methods
Plant materials
Shoot induction experiments
Adventitious shoot elongation, rooting, and acclimatization of regenerated plants
Statistical analysis
Results and Discussion
Effects of explant type and cytokinin type on adventitious shoot induction
Adventitious shoot elongation, rooting, and acclimatization of regenerated plants
Conclusion
Acknowledgements
References

키워드

저자

• Jung Min Kim [ Division of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong 30129, Republic of Korea ]
• Hyun Min Lee [ Division of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong 30129, Republic of Korea ]
• Byung Sun Park [ Division of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong 30129, Republic of Korea ]
• Ha Na Lee [ Division of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong 30129, Republic of Korea ]
• Jae Ik Nam [ Division of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong 30129, Republic of Korea ]
• Chang Ho Ahn [ Division of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong 30129, Republic of Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634