Earticle

초록

영어

Identifying wood species from archaeological artifacts provides crucial information for understanding ancient technological capabilities, resource utilization patterns, paleovegetation, environmental changes, and societal interactions. While traditional microscopical anatomy analysis and common genetic markers (rbcL, rpoB, matK, atp, and 18S) for general plants exhibit limitations in archaeological samples due to DNA degradation and contamination, this study employed the chloroplast trnL gene with shorter sequences for wood identification. Metabarcoding analysis was performed on ancient DNA extracted from wooden components of bronze artifacts (乙-shaped bronze implement, Tubular bronze implement, and Twin-bird Shaped Pommel) excavated from the early Iron Age Namyangju site, targeting the trnL (UAA) intron P6 loop. Using our classifier with reference database constructed from NCBI GenBank and expanded plant database, the analysis identified eight plant families (Asteraceae, Brassicaceae, Convolvulaceae, Marantaceae, Rosaceae, Fabaceae, Poaceae, and Amaryllidaceae). Notably, Rosaceae showed significant presence across all samples (乙-shaped bronze implement: 33.05%, Tubular bronze implement 1: 3.17%, Tubular bronze implement 2: 29.09%, Twin-bird Shaped Pommel: 45.2%), suggesting the use of native woody Rosaceae species. This genetic approach successfully refined the identification of previously unspecified “broadleaf tree/hardwood” specimens to the family level, demonstrating its effectiveness as a complementary method in archaeological wood identification. The results not only provide insights into ancient wood resource utilization patterns but establish a methodological framework for future archaeobotanical studies using ancient DNA analysis.

목차

ABSTRACT
1. INTRODUCTION
2. MATERIALS AND METHODS
2.1. W ood s amples and a ncient D NA ( aDNA) purification for next-generation sequencing
2.2. Library preparation, quality control, and DNA Sequencing
2.3. Bioinformatic analysis of metabarcoding data
3. RESULTS
3.1. Initial analysis and sample characterization
3.2. DNA analysis of archaeological wood samples
3.3. Overview of metabarcoding analysis pipeline
3.4. Performance evaluation of taxonomic classification
3.5. Taxonomic composition and native woody plant identification
4. DISCUSSION
5. CONCLUSION
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Donghyeok Jo [ School of Biological Sciences and Technology, College of Natural Sciences, Chonnam National University, Gwangju 61186, Korea ]
• Seung Hee Baek [ Hangang Institute of Cultural Heritage, Bucheon 14502, Korea ]
• Chungoo Park [ School of Biological Sciences and Technology, College of Natural Sciences, Chonnam National University, Gwangju 61186, Korea ]
• Pyung Won Kang [ Hangang Institute of Cultural Heritage, Bucheon 14502, Korea ]
• Sook Chung Shin [ Hangang Institute of Cultural Heritage, Bucheon 14502, Korea ]
• Lee Hyun-Gwan [ PennBIT Co. Ltd., Gwangju 61062, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  보존과학회지 [Journal of Conservation Science]

• 간기

  연5회

• pISSN

  1225-5459

• eISSN

  2287-9781

• 수록기간

  2003~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 602 DDC 700