Earticle

초록

영어

Aging is associated with declines in memory function and significant change in gut microbiota. In this study, we investigated how exercise affects age-related memory decline and inflammation, and gut microbi-ota diversity. Bl6 mice were divided into control, control and exercise, old, and old and exercise groups. Treadmill exercise was performed once a day, 5 days a week for 8 consecutive weeks. Short-term memo-ry was assessed using step-through test and spatial learning memory was assessed using Morris water maze task. Enzyme-linked immuno-sorbent assay was performed for the proinflammatory cytokines, tumor necrosis factor (TNF)-α and interleukin (IL)-6, in the hippocampus. West-ern blot analysis was conducted for the neurotrophic factors, brain- derived neurotrophic factor (BDNF) and tyrosine kinase B (TrkB), in the hippocampus. In addition, fecal samples were collected for sequencing and metagenomic analysis. Old rats showed decline in short-term mem-ory and spatial learning memory. Increment of TNF-α and IL-6 concen-tration with decrement of BDNF and TrkB expression were observed in the old rats. Decreased diversity of gut microbiota composition and de-creased beneficial gut microbiota were found in the old rats. However, treadmill exercise improved short-term memory, decreased TNF-α and IL-6 concentration, and increased BDNF and TrkB expression in the old rats. Treadmill exercise also increased the diversity of gut microbiota composition and affected the increase of beneficial gut microbiota in the old rats. In conclusion, treadmill exercise reduced age-related in-flammatory markers and effectively improved memory decline while enhancing the diversity and abundance of beneficial gut microbiota.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Experiment animals and groups
Exercise protocol
Step-through test
Morris water maze task
Enzyme-linked immunosorbent assay for proinflammatory cytokines
Western blot analysis for BDNF and TrkB
Collection of fecal samples and sequencing/metagenomic analysis
Data analysis
RESULTS
Short-term memory and spatial learning memory
Concentration of TNF-α and IL-6
Expression of BDNF and TrkB
Microbiome in the gut microbiota
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Sang-Seo Park [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Si-Hyeon Park [ College of Culture and Sports, Division of Global Sport Studies, Korea University, Sejong, Korea ]
• Hyun-Tae Jeong [ College of Culture and Sports, Division of Global Sport Studies, Korea University, Sejong, Korea ]
• Mal-Soon Shin [ College of Culture and Sports, Division of Global Sport Studies, Korea University, Sejong, Korea ]
• Myung-Ki Kim [ College of Culture and Sports, Division of Global Sport Studies, Korea University, Sejong, Korea ]
• Bo-Kyun Kim [ Department of Emergency Technology, College of Health Science, Gachon University, Incheon, Korea ]
• Hye-Sun Yoon [ Department of Pediatrics, Eulji Hospital, Eulji University School of Medicine, Seoul, Korea ]
• Sang-Hoon Kim [ Department of Neurosurgery, Rutgers Robert Wood Johnson Medical School, The Stat University of New Jersey, Piscataway, NJ, USA ]
• Tae-Woon Kim [ Department of Human Health Care, Gyeongsang National University, Jinju, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613