Enrofloxacin is a fluoroquinolone-based synthetic antimicrobial agent that exhibits potent bactericidal effects against gram-positive and gram-negative bacteria that infect aquatic animals. Thus, it is effective in treating bacterial infections in fish. However, antibiotic (enrofloxacin) residues in fish food or fish feed can threaten to human health in association with the development of antibiotic-resistant bacteria. In this case, the dead fish that may have included enrofloxacin residues was turned into fish feed and then sold to fish farmers. A total of 61 fish feed was asked to be examined for the presence of enrofloxacin. Therefore, this present study aimed to analyze enrofloxacin residues in fish feed by liquid chromatography quadrupole time-of-flight mass spectrometry(LC-QTOF/MS). For sample pretreatment, the QuEChERS(Quick, Easy, Cheap, Effective, Rugged, and Safe) method was applied to extract enrofloxacin from the sample and minimize matrix effects. As a result, enrofloxacin was detected in all the fish feed samples.
목차
Abstract Ⅰ. 서론 Ⅱ. 실험방법 1. 시약 및 재료 2. 시료 전처리 3. LC-QTOF/MS 분석조건 Ⅲ. 결과 및 고찰 Ⅳ. 사사 Ⅴ. 참고문헌
법과학 분야는 사회정의 구현에 있어 크나큰 가치가 있음에도 불구하고 우리나라에서는 이 분야에 대한 인식이 미흡하여 선진 외국에 비해 침체되어 있는 실정이다. 이에 우리나라에서도 법과학 분야와 관련 있는 학계, 연구기관, 수사기관 등 유관 단체들로 구성된 한국 법과학회를 창립하여 이 분야를 활성화 시켜 과학수사를 한층 더 발전시키기 위함을 목적으로 한다.