Earticle

초록

영어

Cannabis is strictly regulated under the Narcotics Control Act in Korea. Urine and hair samples are commonly used as biological samples to prove cannabis abuse. In general, to determine whether cannabis has been abused, 11-nor-9-carboxy -tetrahydrocannabinol (Δ9-THC-COOH), a major metabolite of cannabis, is analyzed to rule out the possibility of external contamination. Recently, 11-nor-8-tetrahydrocannabinol (Δ8-THC), an isomer of 11-nor-9-tetrahydrocannabinol (Δ9-THC) synthesized with cannabidiol (CBD), has also been abused in the form of electronic cigarettes, and its metabolite is 11-nor-8-carboxytetrahydrocannabinol (Δ8-THC-COOH). We attempted to analyze these cannabis metabolites in the nails to determine whether the subjects smoked cannabis. Method validation was performed using a Sciex 6500 LC/MS/MS for Δ8-THC-COOH and Δ 9-THC-COOH (THC-COOHs) in nails. The instrument conditions for THC-COOHs analysis were the same as those used for hair analysis, a column switching LC–ESI-MS 3 method. THC-COOHs were extracted by liquid-liquid extraction from digested nails with 1 N NaOH. The quantification of THC-COOHs and the identification of THC-COOH-d3 were performed using multistage fragmentation (MS3) with two transitions for THC-COOHs (m/z 343.1→299.1→245.2 and m/z 343.1→299.1→191.1) and one transition for THC-COOH- d3 (m/z 346.1→302.2→248.1). The quantifier ions are m/z 245.2 and 248.1. The calibration curve ranged from 0.05 to 20 pg/mg, R2 exceeded 0.999, and the LOD and LOQ of both components were 0.05 and 0.1 pg/mg, respectively. The accuracy and precision, matrix effect, recovery, and process efficiency were all within 15% of the CV values. Method validation results for THC-COOHs analysis for nails were not significantly different from those of hair, including the LOD and LOQ results. (Table 4.) Only one of the three authentic nail samples showed a positive result for Δ9-THC-COOH at a concentration of 12.29 pg/mg. These results indicate that since nails and hair can be analyzed under similar conditions, they may be useful alternatives to each other when collecting evidence of cannabis abuse.

목차

Abstract
Ⅰ. Introduction
1. Case History
Ⅱ. Materials and instruments
1. Chemicals
2. Instruments
3. Sample collection and preparation
4. Method validation
Ⅲ. Results
1. Method validation results
2. Sample analysis results
Ⅳ. Discussion and conclusion
Ⅴ. Acknowledgements
Ⅵ. References

키워드

저자

• Juseon Lee [ Forensic Toxicology Division, National Forensic Service, Wonju, Korea ] Corresponding Author
• Byungseok Cho [ Forensic Toxicology and Chemistry Division, Daejeon Institute, Daejeon, Korea ]
• Dongeun Park [ Forensic Toxicology Division, National Forensic Service, Wonju, Korea ]
• Bohye Kim [ Forensic Toxicology Division, National Forensic Service, Wonju, Korea ]
• Hyeon Jegal [ Forensic Toxicology and Chemistry Division, Busan Institute, Yangsan, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국법과학회지 [Korean Journal of Forensic Science]

• 간기

  반년간

• pISSN

  1598-0715

• 수록기간

  2000~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 367 DDC 347