Earticle

초록

영어

BACKGROUND: Pleurotus ostreatus, oyster mushroom, is a popular edible mushroom cultivated world-wide and it also belongs to white-rot fungi that can degrade plant cell wall lignin effectively. To develop an efficient strategy for molecular breeding in P. ostreatus, molecular genetic tools have been developed through my 30-years investigation with wonderful colleagues from domestic and foreign countries. One of the most fruitful and newest international collaborations is that with Korean researchers. In this talk, I would like to introduce some recent results of an international collaboration with Mushroom Division, NIHHS, Rural Development Administration, Republic of Korea. RESULTS: We have successfully introduced a genome editing technology into P. ostreatus using CRISPR/Cas92. To establish a non-genetically modified (non-GM) genome editing in mushroom fungi, we set sail for developing a protocol for CRISPR/Cas9 without foreign DNA integration in the edited strain. In addition, to avoid health and environmental problems caused by spores, we selected and knocked-out candidate genes essential for spore formation using CRISPR/Cas9 technique. Three new genes essential for spore formation were found and sporeless cultivars by disrupting one of these genes were isolated. CONCLUSION: The fundamental technology of non-GM molecular breeding can be used for valuable objectives including high-value-added edible mushrooms, efficient lignin-degrading strains with high potential for bioremediation and non-existent strains su

키워드

저자

• Yoichi Honda [ Div. of Forest and Biomaterial Science, Graduate School of Agriculture, Kyoto University Kitashirakawa-oiwakecho, Sakyoku, Kyoto, 6068502, Japan ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  버섯 [Mushroom]

• 간기

  반년간

• 수록기간

  1997~2025

• 십진분류

  KDC 525 DDC 635