Earticle

현재 위치 Home

Original Article

Influence of aqueous extract of Annona muricata leaves in Tris-egg yolk extender on storage of spermatozoa from West African Dwarf goat (Capra hircus )

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Journal of Animal Reproduction and Biotechnology 바로가기
  • 통권
    Volume. 39 No. 3 (2024.09)바로가기
  • 페이지
    pp.179-193
  • 저자
    Mohamadou Adamou, Dongmo Nguedia Arius Baulland, Ngo Bahebeck Pierrette, Tchoffo Herve, Chongsi Margaret Mary Momo, Noudjio Kezeter Claude, Nnanga Germaine Estelle Mvondo, Ngwemetah Nathalie, Adamu Yusufa Njeiri, Ngoula Ferdinand
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A456755

※ 기관로그인 시 무료 이용이 가능합니다.

4,800원

원문정보

초록

영어
Background: Because oxidative stress can induce decreased quality of caprine semen during the storage, there has been limitation for the use of stored semen in the assisted reproductive technologies. The present study, therefore, assesses the potential of Annona muricata (A. muricata ) to reduce semen storage associateddamages. Methods: Semen was collected by electro-ejaculation from ten bucks, and extended with Tris-egg yolk (TEY) supplemented with A. muricata leaf aqueous extract (SAE) at 20 (SAE20), 40 (SAE40), and 80 (SAE80) μg/mL. Sperm variables including motility, motion characteristics, viability, membrane functionality, and DNA integrity were assessed at different storage periods (6, 24, 48, and 72 hr). In addition, oxidative stress indicators in the extender supplemted with SAE were also assessed for each group. Results: By adding SAE at 80 μg/mL in TEY, the storage of goat buck semen was improved, resulting in reduced loss of sperm motility, viability, DNA fragmentation, and membrane integrity during chilled storage at 4℃ for up to 72 hr. In addition, enrichment of TEY extender with SAE significantly (p < 0.05) reduced malondialdehyde, an indicator of oxidative stress, compared to the negative control. Conclusions: Supplementation of SAE in TEY extender can reduce buck spermatozoa liquid storage associated damages due to oxidative stress.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Study site
Annona muricata (Soursop) leaves harvesting and preparation of aqueous extract
Animals and semen collection
Extender preparation and semen processing
Assessment of sperm viability
Assessment of sperm functional membrane integrity
Assessment of sperm DNA integrity
Assessment of sperm motility
Assesment of total peroxidases
Assesment of lipid peroxidation
Assessment of superoxide dismutase activity
Assessment of catalase activity
Statistical analysis
RESULTS
Effects of SAE on buck’s sperm total motility, progressive motility, and local motility
Simple interactions of time and doses of SAE on buck’s sperm total motility, progressive motility, and local motility
Effects of SAE on buck’s sperm motion characteristics
Simple interactions of time and doses of SAE on buck’s sperm motion characteristics
Effects of SAE on buck’s sperm viability, plasma membrane and DNA integrity
Simple interactions of time and doses of SAE on buck’s sperm viability, plasma membrane and DNA integrity
Effects of incorporating SAE in TEY extender on lipid peroxidation biomarkers content in buck’s semen
Simple interactions of time and doses of SAE in TEY extender on oxidative stress biomarkers content in buck’s semen
DISCUSSION
CONCLUSION
REFERENCES

키워드

Annona muricata buck semen liquid semen storage storage-associated damages Tris-egg yolk extender

저자

  • Mohamadou Adamou [ Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang 188, Cameroon/Institute of Agricultural Reseach for Development, Mbalmayo Agricultural Research Center, Yaounde 2067, Cameroon ] Corresponding Author
  • Dongmo Nguedia Arius Baulland [ Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang 188, Cameroon ]
  • Ngo Bahebeck Pierrette [ Institute of Agricultural Reseach for Development, Mbalmayo Agricultural Research Center, Yaounde 2067, Cameroon ]
  • Tchoffo Herve [ Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang 188, Cameroon ]
  • Chongsi Margaret Mary Momo [ Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang 188, Cameroon ]
  • Noudjio Kezeter Claude [ Institute of Agricultural Reseach for Development, Mbalmayo Agricultural Research Center, Yaounde 2067, Cameroon ]
  • Nnanga Germaine Estelle Mvondo [ Institute of Agricultural Reseach for Development, Mbalmayo Agricultural Research Center, Yaounde 2067, Cameroon ]
  • Ngwemetah Nathalie [ Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang 188, Cameroon ]
  • Adamu Yusufa Njeiri [ College of Technology, University of Bamenda, Bamenda 39, Cameroon ]
  • Ngoula Ferdinand [ Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang 188, Cameroon ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Journal of Animal Reproduction and Biotechnology
  • 간기
    계간
  • pISSN
    2671-4639
  • eISSN
    2671-4663
  • 수록기간
    2019~2026
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Journal of Animal Reproduction and Biotechnology Volume. 39 No. 3

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.