Earticle

초록

영어

Background: Typical difficulties encountered during in vitro fertilization (IVF) to produce embryos in pigs include poor pronucleus formation and poor-quality fertilized embryos because of high polysperm invasion. In this study, we evaluated the effects of supplementation with apple seed extract (ASE) and coculture systems on porcine in vitro-fertilized embryo culture. Methods: Slaughterhouse-derived ovaries were used to obtain cumulus-oocyte complexes (COCs). COCs were conventionally used to perform IVF. We examined the differences in apoptosis and metabolism during development following addition of ASE to normal culture and coculture systems. Matrix metalloproteinases (MMPs), cell development-related factors, and apoptotic proteins were compared in porcine embryos produced under different conditions. Results: The expression of genes related to insulin-like growth factor (IGF) signaling was increased in the coculture system. In the ASE group, early apoptosis and necrosis were reduced in fertilized embryos and the late survival rate increased. Supplementation of the coculture system with ASE led to increased expression of BCL-2 and decreased expression of Casp-3 in the cytoplasm, thereby lowering the apoptosis rate and inducing MMP expression. In addition, compared with the extract-supplemented group in normal culture, the activity of MMP-2 decreased in the coculture system supplemented with ASE, activity of MMP-9 increased, and the expression of dynactin p62 and BrdU in the cytoplasm was higher than that in the other groups. Conclusions: The coculture system increased the activity of the embryonic cytoplasm compared with the non-coculture system. Supplementation with ASE may induce cell activity and inhibit the expression of apoptotic factors.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Oocyte maturation
Sperm processing and IVF
In vitro culture
Gelatin zymography
ELISA
Immunofluorescence
Statistical analysis
RESULTS
Analysis of embryonic cell death factor activity according to ASE treatment
Action of cytoplasmic and cell cycle activators
Analysis of activity and expression control of MMPs in embryonic cells
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Min-Jee Oh [ General Graduate School of Animal Life Convergence Science, Hankyong National University, Institute of Applied Humanimal Science, Hankyong National University, Anseong 17579, Korea ]
• Baasanjav Batmunkh [ General Graduate School of Animal Life Convergence Science, Hankyong National University, Anseong 17579, Korea ]
• Ji-Yeon Mo [ School of Animal Life Convergence Science, Hankyong National University, Anseong 17579, Korea ]
• Sang-Hwan Kim [ General Graduate School of Animal Life Convergence Science, Hankyong National University, School of Animal Life Convergence Science, Hankyong National University, Institute of Applied Humanimal Science, Hankyong National University, Anseong 17579, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636