Earticle

초록

영어

Background: The clinical application of canine mesenchymal stem cells (MSCs) necessitates efficient and safe culture methods to produce large quantities of cells. Traditionally, fetal bovine serum (FBS) has been used for MSC expansion, but it carries risks such as contamination and adverse immune responses. Methods: In this study, we investigate the efficacy and efficiency of canine allogeneic serum as an effective alternative to FBS for the in vitro culture of canine MSCs. We measured the population doubling time of canine MSCs in allogeneic serum conditions and utilized qRT-PCR, flowcytometric analysis, and cellular staining/color-metric assay for investigating its effects on cellular senescence during long-term culture and the expression of key pluripotency-related transcriptomes. Results: Our findings demonstrate that canine MSCs cultured with allogeneic serum exhibited enhanced proliferation rates, reduced cellular senescence, and lower apoptosis levels compared to those cultured with FBS. Additionally, the expression of key pluripotency-related transcription factors, including Oct4, Sox2, and Nanog, was increased in canine MSCs cultured with allogeneic serum. Conclusions: These results highlight the potential of canine allogeneic serum to provide a safer and more effective culture environment, supporting the large-scale expansion and maintenance of canine MSCs for clinical applications.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Isolation and culture of MSCs derived from human and canine fat tissues
Preparation of allogenic serum
The measurement of population doubling time (PDT)
Senescence associated β-galactosidase assay
The analysis of cell apoptosis
Quantitative real time-PCR (qRT-PCR) analysis
Statistical analysis
RESULTS
Differential in vitro growth capacity in human and canine mesenchymal stem cells
Effect of allogeneic serum on in vitro expansion of canine MSCs
Allogeneic serum reduces cellular senescence of canine MSCs in vitro culture conditions
Expression of pluripotency-related transcription factors in canine MSCs cultured with FBS versus allogeneic serum
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Yong-ho Choe [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Sang-Yun Lee [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Young-Bum Son [ Department of Obstetrics, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea ]
• Won-Jae Lee [ College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ]
• Hyeonjeong Lee [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Chan-Hee Jo [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Seong-Ju Oh [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Tae-Seok Kim [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Chae-Yeon Hong [ College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea ]
• Sung-Lim Lee [ College of Veterinary Medicine, Gyeongsang National University, Research Institute of Life Sciences, Gyeongsang National University, Jinju 52828, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636