Earticle

초록

영어

Dalbergia sissoo and Dalbergia latifolia are the two most important timber wood species that are indigenous to the Indo-Pak subcontinent producing very high-quality timber. Its wood is used to produce high-quality furniture. Due to overexploitation and dieback disease, their production is seriously hampered and threatens their genetic diversity. Several ecotypes are tolerant to dieback thus offering an opportunity to develop a superior hybrid for the establishment of plantations for hybrid seed production. Hybrid evaluation can only be done by using DNA markers such as SSR markers. Cross-specific amplification of SSR markers is a cost-effective way of producing DNA markers for species lacking genetic information. Here, we report for the first-time successful cross-amplification of SSR markers in Dalbergia latifolia and added new SSR markers in D. sissoo. Cross-species amplification resulted in 13 successful SSR markers in D. latifolia and an addition of 14 markers in D. sissoo of expected sizes. Six SSR markers were further selected randomly to validate the breeding behavior of both species. A diverse DNA profile of seed progenies matched to different pollen donors deviated from the same mother suggested cross-pollination is the most likely mechanism of seed production in D. sissoo and D. latifolia. However, the results must be validated by using a large sample size and through controlled pollinations. SSR markers thus developed will be useful in the conservation and development of superior hybrids for sustainable development and production of commercial populations in Dalbergia sissoo and D. latifolia.

목차

Abstract
Introduction
Materials and Methods
PCR assay
Mating system analysis
Data analysis
Results and Discussion
Conclusion
Acknowledgements
References

키워드

저자

• Asif Javed Muhammad [ Department of Forestry and Range Management, Faculty of Agriculture, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan/Department of Centre for Advanced Studies, Agriculture Food Security (CAS-AFS), University of Agriculture Faisalabad, Faisalabad 38000, Pakistan ] Corresponding Author
• Izhar-Ul-Haq [ Department of Forestry and Range Management, Faculty of Agriculture, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan/Department of Centre for Advanced Studies, Agriculture Food Security (CAS-AFS), University of Agriculture Faisalabad, Faisalabad 38000, Pakistan ]
• Nauman Ghafoor [ Department of Forestry and Range Management, Faculty of Agriculture, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan/Department of Centre for Advanced Studies, Agriculture Food Security (CAS-AFS), University of Agriculture Faisalabad, Faisalabad 38000, Pakistan ]
• Fazal-Ur-Rehman [ Department of Forestry and Range Management, Faculty of Agriculture, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan/Department of Centre for Advanced Studies, Agriculture Food Security (CAS-AFS), University of Agriculture Faisalabad, Faisalabad 38000, Pakistan ]
• Atif Ali [ Department of Forestry and Range Management, Faculty of Agriculture, University of Agriculture Faisalabad, Faisalabad 38000, Pakistan ]
• Zia Bilal Muhammad [ Division of Genetics, Pakistan Forest Institute, Peshawar 25130, Pakistan ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634