Earticle

초록

영어

Fluorescent bacteria were isolated from sporocarps that browned into various mushrooms during survey at places of the production in Korea. We examined the pathogenicity, biodiversity, and genetic characteristics of the 19 strains identified as Pseudomonas tolaasii by sequence analysis of 16S rRNA and White Line Assay. The results emphasize the importance of rpoB gene system, fatty acid profiles, specific and sensitive PCR assays, and lipopeptide detection for the identification of P. tolaasii. As a result of these various analyses, 17 strains (CHM03~CHM19) were identified as P. tolaasii. The phylogenetic analysis based on the 16S rRNA gene showed that all strains were clustered closest to P. tolaasii lineage, two strains (CHM01, CHM02) were not identified as P. tolaasii and have completely different genetic characteristics as a result of fatty acids profile, specific and sensitive PCR, lipopetide detection, rpoB sequence and REP-PCR analysis. Pathogenicity tests showed 17 strains produce severe brown discolouration symptoms to button mushrooms and watersoaking of sporophore tissue within three days after inoculation. But two strains did not produce discolouration symptoms. Therefore, these two strains will be further investigated for correct species identification by different biological and molecular characteristics.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Bacterial culture conditions
White Line Assay and Lipopeptide detection
Genomic DNA isolation and sequencing
Molecular genetic analysis
Biochemical assays
In vitro antagonism experiments and necrosis test
Pathogenicity test
RESULTS AND DISCUSSION
Biodiversity of the bacterial isolates
Molecular genetic analysis of isolates
Detection of lipopeptide metabolites
Pathogenicity and necrotic properties
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Chan-Jung Lee [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong 27709, Korea ] Corresponding Author
• Hye-Sung Park [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong 27709, Korea ]
• Seong-Yeon Jo [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong 27709, Korea ]
• Gi-Hong An [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong 27709, Korea ]
• Ja-Yun Kim [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong 27709, Korea ]
• Kang-Hyo Lee [ Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, Eumseong 27709, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국버섯학회지 [Journal of MUSHROOMS]

• 간기

  계간

• pISSN

  1738-0294

• eISSN

  2288-8853

• 수록기간

  2003~2025

• 등재여부

  KCI 등재

• 십진분류

  KDC 525 DDC 635