Earticle

초록

영어

Background: Despite its anticancer activity, cisplatin exhibits severe testicular toxicity when used in chemotherapy. Owing to its wide application in cancer therapy, the reduction of damage to normal tissue is of imminent clinical need. In this study, we evaluated the effects of catechin hydrate, a natural flavon-3-ol phytochemical, on cisplatin-induced testicular injury. Methods: Type 2 mouse spermatogonia (GC-1 spg cells) were treated with 0-100 μM catechin and cisplatin. Cell survival was estimated using a cell proliferation assay and Ki-67 immunostaining. Apoptosis was assessed via flow cytometry with the Dead Cell Apoptosis assay. To determine the antioxidant effects of catechin hydrate, Nrf2 expression was measured using qPCR and CellROX staining. The anti-inflammatory effects were evaluated by analyzing the gene and protein expression levels of iNOS and COX2 using qPCR and immunoblotting. Results: The 100 μM catechin hydrate treatment did not affect healthy GC-1 spg cells but, prevented cisplatin-induced GC-1 spg cell death via the regulation of anti-oxidants and inflammation-related molecules. In addition, the number of apoptotic cells, cleaved-caspase 3 level, and BAX gene expression levels were significantly reduced by catechin hydrate treatment in a cisplatin-induced GC-1 spg cell death model. In addition, antioxidant and anti-inflammatory marker genes, including Nrf2 , iNOS, and COX2 were significantly downregulated by catechin hydrate treatment in cisplatintreated GC-1 cells. Conclusions: Our study contributes to the opportunity to reintroduce cisplatin into systemic anticancer treatment, with reduced testicular toxicity and restored fertility.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
1. Cell culture and treatment
2. Cell proliferation assay
3. Immunostaining
4. Flow cytometry analysis
5. RNA extraction and quantitative PCR
6. Immunoblotting
7. Statistical analysis
RESULTS
1. Catechin hydrate protects against cisplatin-induced germ cell damage
2. Pro-apoptotic protein expression levels in GC-1spg cells after cispatin and catechin treatment
3. Antioxidant and anti-inflammatory effects of catechin hydrate in cisplatin-induced GC-1 spg cell damage
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Hyeon Woo Shim [ Department of Animal Biotechnology, College of Life Science, Sangji University, Wonju 26339, Korea ]
• Won-Yong Lee [ Department of Livestock, Korea National University of Agriculture and Fisheries, Jeonju 54874, Korea ]
• Youn-Kyung Ham [ Department of Animal Science, College of Life Science, Sangji University, Wonju 26339, Korea ]
• Sung Don Lim [ Department of Plant Life and Resource Science, College of Life Science, Sangji University, Wonju 26339, Korea ]
• Sun-Goo Hwang [ Department of Plant Life and Resource Science, College of Life Science, Sangji University, Wonju 26339, Korea ]
• Hyun-Jung Park [ Department of Animal Biotechnology, College of Life Science, Sangji University, Wonju 26339, Korea ] Corresponding Author

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636