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This study reported the novel use of fallen leaf extract as a microbial culture media for the first time. Extract from hardwood fallen leaves (HLE) was prepared under high temperature and pressure conditions and then supplemented with specific nutrients. The growth of four industrially significant prokaryotes on the HLE-based media was measured and compared with that on enriched media (Luria-Bertani, LB). Notably, supplementing HLE with only 0.5 g of yeast extract and 1 g tryptone per liter showed a similar growth rate of Pseudomonas chlororaphis compared to standard LB media. Overall, the HLE media developed in this study offers a sustainable and cost-effective approach to microbial media production, capitalizing on the valorization of forest waste.
목차
Abstract Introduction Materials and Methods Media preparation using hardwood fallen leaves(HLE) Microbial growth in HLE media Statistical analysis Results and Discussion Component analysis of the HLE Prokaryotic growth on HLE Supplementation of HLE media Conclusions Acknowledgements References
Yongtae Yu [ Department of Forest Biomaterials Engineering, College of Forest and Environmental Science, Kangwon National University ]
Hyunjun Ko [ Department of Forest Biomaterials Engineering, College of Forest and Environmental Science, Kangwon National University ]
Corresponding Author