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Carbon dots attached nanocellulose as a recyclable nanozyme for colorimetric biosensing

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    강원대학교 산림과학연구소 학술대회 바로가기
  • 통권
    2022 International symposium of Institute of Forest Science for the 40th Anniversary of College of Forest and Environment Science (2022.10)바로가기
  • 페이지
    pp.67-67
  • 저자
    Rajkumar Bandi, Ramakrishna Dadigala, Song-Yi Han, Jeong-ki Kim, Gu-Joong Kwon, Seung-Hwan Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A450443

원문정보

초록

영어
Carbon dots (CDs) are widely employed as peroxidase mimics in biosensing. However, as colloidal nanozymes, CDs suffer from the drawbacks of substrate induced aggregation and difficulties in recovery and reuse. Nanocellulose is proposed as a support material to overcome these drawbacks and two immobilization strategies were explored here. In first strategy, peroxidase mimicking Fe-doped CDs (FeCDs) were immobilized on TEMPO-oxidized cellulose nanofibrils (TOCNF) via physical entrapment involving hydrogen bonding interactions. Peroxidase activity of the composite nanopaper was evaluated using TMB as chromogenic substrate and applied to the colorimetric detection of H2O2 and glucose. Under optimal conditions, this method afforded linear ranges of 6– 42 μM and 10–70 μM and detection limits of 0.93 and 1.73 μM for the H2O2 and glucose, respectively. In another strategy, amine functionalized, Fe, N-doped CDs (FeNCDs) were immobilized on dialdehyde cellulose nanofibrils (DACNF) via Schiff base reaction and reductive amination. DACNF served as an excellent matrix for FeNCDs to retain the fluorescence and peroxidase activities. Colorimetric assays based on ABTS oxidation offered linear ranges of 10–80 μM and 50–400 μM and detection limits of 0.70 and 3.72 μM for H2O2 and cholesterol respectively. Further the nanocomposite presented a long shelf life and excellent reusability for 10 cycles. Overall, nanocellulose acted as a great support material for the colloidal nanozyme that prevented them from aggregation and made them easy to recycle and reuse.

키워드

nanocellulose carbon dots peroxidase mimics glucose detection cholesterol detection

저자

  • Rajkumar Bandi [ The institute of Forest Science, Kangwon National University, Republic of Korea ]
  • Ramakrishna Dadigala [ The institute of Forest Science, Kangwon National University, Republic of Korea ]
  • Song-Yi Han [ The institute of Forest Science, Kangwon National University, Republic of Korea ]
  • Jeong-ki Kim [ Department of Forest Biomaterials & Engineering, Kangwon National University, Republic of Korea ]
  • Gu-Joong Kwon [ The institute of Forest Science, Kangwon National University, Kangwon Institute of Inclusive Technology, Kangwon National University, Republic of Korea ]
  • Seung-Hwan Lee [ The institute of Forest Science, Kangwon National University; Department of Forest Biomaterials & Engineering, Kangwon National University; Kangwon Institute of Inclusive Technology, Kangwon National University, Republic of Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    강원대학교 산림과학연구소 학술대회
  • 간기
    부정기
  • 수록기간
    2017~2024
  • 십진분류
    KDC 526 DDC 634

이 권호 내 다른 논문 / 강원대학교 산림과학연구소 학술대회 2022 International symposium of Institute of Forest Science for the 40th Anniversary of College of Forest and Environment Science

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