KNU IFS 2018 Annual International Symposium of Institute of Forest Science (2018.09)바로가기
페이지
pp.103-103
저자
Jihyeon Lee, Hanjin Yu, Kwang Suk Lim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A450215
원문정보
초록
영어
To effectively delivery of nucleic acids into the cells, various gene delivery systems are required. They protect nucleic acids against immune response, serum, and elimination mechanism. Nonviral gene delivery systems have low immune response, low side effect and temporary transfection compared with viral systems. Cellulose nanocrystal (CNC) is widely used as biomedical materials because of its good mechanical properties due to their lower weight, bbiocompatibility and biodegradability. In this study, we determined that CNC-Zinc/DNA combination systems (CZD cluster) could be deliver nucleic acids into the cells. CZD cluster successfully retarded migration of nucleic acids in agarose gel and enhanced DNA transfection efficiency into the cells. CZD cluster was spontaneously formed without any chemical reaction. This cluster systems could be used to deliver any plasmid DNA or siRNA in the cells and tissues without the requirement for a specific modification of the nucleic acids.