KNU IFS 2018 Annual International Symposium of Institute of Forest Science (2018.09)바로가기
페이지
pp.98-98
저자
Da-Jin Son, Dong-Min ParK, Deok-Ho Kwon, Jae-Bum Park, Suk-Jin Ha
언어
영어(ENG)
URL
https://www.earticle.net/Article/A450210
원문정보
초록
영어
Catechin belonging to the tannins are found in various kinds of tea, and are mainly contained in green tea. It has a strong reducing effect and have antioxidant, antiviral, antibacterial effect as well. In addition, derivatives produced from catechin degradation are known as high value-added substances. Previously, we isolated two bacteria capable of degrading catechin from soil. These two bacteria were identified as Enterobacter sp. Several genes were cloned to identify catechin degrading enzymes from two isolated bacteria. Using cloned genes, catechin degrading enzyme overexpression systems were constructed with pET-21a vector. As results of overexpression using a recombinant E. coli, a soluble from enzyme was overexpressed. This enzyme exhibited catechin degradation activity after purification using 6His tag.
Da-Jin Son [ Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ]
Dong-Min ParK [ Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ]
Deok-Ho Kwon [ Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ]
Jae-Bum Park [ Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ]
Suk-Jin Ha [ Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ]
Corresponding author