KNU IFS 2018 Annual International Symposium of Institute of Forest Science (2018.09)바로가기
페이지
pp.97-97
저자
Jae-Bum Park, Jin-Seong Kim, Deok-Ho Kwon, Jin-Ho Seo, Dae-Hyuk Kweon, Suk-Jin Ha
언어
영어(ENG)
URL
https://www.earticle.net/Article/A450209
원문정보
초록
영어
Due to increased environmental concerns and needs for energy security, biofuel productions have become attractive alternatives to the fossil fuels. The production of biofuels from edible starch were suffer from ethical problems of using food resources. Biofuels production using non-edible cellulosic biomass has been attempted to solve those problems. Xylose, one of the main components of cellulosic biomass, can be converted to xylitol which has been known as a valuable synthetic building block. In this study, K. marxianus KCTC17555ΔURA3 strain was engineered by multi-copy random integration of the mutant KmXYL1 (mKmXYL1) gene from K. marxianus 36907-FMEL1. When fermentation experiments were performed using xylose as a sole carbon source at 40°C, K. marxianus 17555-JBP2 produced 53.17 g·L-1 of xylitol with 0.73 g·g-1 yield and 0.55 g·L-1·h-1 productivity. In conclusion, K. marxianus 17555-JBP2 showed 190% and 300% improvement of xylose consumption rate and xylitol production rates, respectively.
Jae-Bum Park [ Department of Bioengineering and Technology, Kangwon National University ]
Jin-Seong Kim [ Department of Bioengineering and Technology, Kangwon National University ]
Deok-Ho Kwon [ Department of Bioengineering and Technology, Kangwon National University ]
Jin-Ho Seo [ Department of Agricultural Biotechnology, Seoul National University, Seoul 152 -742, Republic of Korea ]
Dae-Hyuk Kweon [ Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea ]
Suk-Jin Ha [ Department of Bioengineering and Technology, Kangwon National University ]
Corresponding Author