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Improvement of K. marxianus strains for efficient xylitol production through the mutant KmXYL1 gene overexpression

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    강원대학교 산림과학연구소 학술대회 바로가기
  • 통권
    KNU IFS 2018 Annual International Symposium of Institute of Forest Science (2018.09)바로가기
  • 페이지
    pp.97-97
  • 저자
    Jae-Bum Park, Jin-Seong Kim, Deok-Ho Kwon, Jin-Ho Seo, Dae-Hyuk Kweon, Suk-Jin Ha
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A450209

원문정보

초록

영어
Due to increased environmental concerns and needs for energy security, biofuel productions have become attractive alternatives to the fossil fuels. The production of biofuels from edible starch were suffer from ethical problems of using food resources. Biofuels production using non-edible cellulosic biomass has been attempted to solve those problems. Xylose, one of the main components of cellulosic biomass, can be converted to xylitol which has been known as a valuable synthetic building block. In this study, K. marxianus KCTC17555ΔURA3 strain was engineered by multi-copy random integration of the mutant KmXYL1 (mKmXYL1) gene from K. marxianus 36907-FMEL1. When fermentation experiments were performed using xylose as a sole carbon source at 40°C, K. marxianus 17555-JBP2 produced 53.17 g·L-1 of xylitol with 0.73 g·g-1 yield and 0.55 g·L-1·h-1 productivity. In conclusion, K. marxianus 17555-JBP2 showed 190% and 300% improvement of xylose consumption rate and xylitol production rates, respectively.

키워드

Thermotolerant yeast Kluyveromyces marxianus Cellulosic biomass Xylose Xylose reductase

저자

  • Jae-Bum Park [ Department of Bioengineering and Technology, Kangwon National University ]
  • Jin-Seong Kim [ Department of Bioengineering and Technology, Kangwon National University ]
  • Deok-Ho Kwon [ Department of Bioengineering and Technology, Kangwon National University ]
  • Jin-Ho Seo [ Department of Agricultural Biotechnology, Seoul National University, Seoul 152 -742, Republic of Korea ]
  • Dae-Hyuk Kweon [ Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea ]
  • Suk-Jin Ha [ Department of Bioengineering and Technology, Kangwon National University ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    강원대학교 산림과학연구소 학술대회
  • 간기
    부정기
  • 수록기간
    2017~2024
  • 십진분류
    KDC 526 DDC 634

이 권호 내 다른 논문 / 강원대학교 산림과학연구소 학술대회 KNU IFS 2018 Annual International Symposium of Institute of Forest Science

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