Earticle

초록

영어

Due to the depletion of fossil fuels, cellulosic biomass has been focused on as an attractive resource. Cellulosic biomass, mainly composed of cellulose and hemicellulose, is enzymatically hydrolyzed to glucose and xylose, respectively. Therefore, it is necessary to develop the strain capable of utilizing both glucose and xylose efficiently for biofuel productions. However, glucose and xylose are consumed sequentially because of catabolite repression by glucose, which cause inefficient utilization of cellulosic biomass. In this study, we performed a directed evolutionary approach using 2-deoxyglucose, the analogue of glucose, to isolate the mutant K. marxianus SBK1 capable of cofermenting glucose and xylose simultaneously. K. marxianus SBK1 exibited improved xylose consumption rate (163%), ethanol yield (19%), and ethanol productivity (67%) as compared to the parental strain. Cofermentations were performed with various concentrations of glucose and 40 g/L of xylose to investigate the effect of catabolite repression by glucose. When the glucose concentrations were increased, the xylose consumption rates decreased gradually. It suggests that K. marxianus SBK1 still showed catabolite repression at high concentrations of glucose although catabolite repression was highly alleviated at low concentrations of glucose.

키워드

저자

• Saet-Byeol Kim [ Department of Bioengineering and Technology, College of engineering, Kangwon National University ]
• Deok-Ho Kwon [ Department of Bioengineering and Technology, College of engineering, Kangwon National University ]
• Jae-Bum Park [ Department of Bioengineering and Technology, College of engineering, Kangwon National University ]
• Suk-Jin Ha [ Department of Bioengineering and Technology, College of engineering, Kangwon National University ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  강원대학교 산림과학연구소 학술대회

• 간기

  부정기

• 수록기간

  2017~2024

• 십진분류

  KDC 526 DDC 634