Earticle

초록

영어

Cinnamomum camphora (Lauraceae) is an evergreen broad-leaved tree and distributed in the south of China and Japan. This species has been widely cultivated in many countries because of its aromatic oils, insect-repellent effects and ornamental value. Despite its vast utilization and cultivation, little is known about its genetic diversity and population genetics. To date, only a few genomic simple sequence repeat (SSR) markers were developed in C. camphora and no genic-SSR primers were available yet. Thus, it is necessary to develop more genic-SSR markers, which will facilitate the genetic studies of Cinanamomum. In this study, a total of 74,289 simple sequence repeats (SSRs) were found in 56,124 unigenes, of which 14,225 unigenes contained more than one SSR locus. Among these SSR loci, the mono-nucleotide repeats were the most frequent, with a frequency of 61.14%, followed by 24.87% di-nucleotide repeats and 12.87% tri-nucleotide repeats. Twenty-one polymorphic SSR markers were developed and validated in 45 camphor trees. The 21 loci were further examined for genetic diversity of 38 provenances, 190 individuals in the whole natural distribution area of camphor tree in China. The results showed that 74 polymorphic sites were detected with average 2.74 polymorphic were detected for each prime. The means of observed and expected heterozygosities were 0.4446 and 0.4419, respectively. Nei's and Shannon’s information index for these populations were 0.4400 and 0.7193, respectively. The genetic similarity coefficient of 38 provenances was between 0.05 and 0.95, with an average of 0.17.The smallest genetic distance is JXWY and JXRJ and the genetic distance between JXTG and GZDZ was the largest. Cluster analysis in 0.88 genetic distance can divide into five groups. Jiangxi provenances clustered into two groups, of which JXAY grouped into one group and other provenances grouped into another group. Other provenances of China grouped three small branches: CQYY and HNHH, GZDZ and SCLZ, and other provenances. The clustering results showed that the genetic components of camphor tree in China had obvious regional features. The analysis of molecular variance exhibited that 77% of genetic variation was observed within the populations. The novel genic-SSR markers will not only benefit genetic diversity analysis and wild resources conservation of C. camphora, but also contribute to exploring the further evolutionary history and genetic differentiation pattern of Cinnamomum.

키워드

저자

• Yongda Zhong [ Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330029, P.R. China ] Corresponding Author
• Zhiting Li [ Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, P.R. China ]
• Shujuan Liu [ Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330029, P.R. China ]
• Aihong Yang [ Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330029, P.R. China ]
• Lipan Liu [ Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330029, P.R. China ]
• Yanqiang Li [ Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330029, P.R. China ]
• Meng Xu [ Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, P.R. China ]
• Faxin Yu [ v | Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330029, P.R. China ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  강원대학교 산림과학연구소 학술대회

• 간기

  부정기

• 수록기간

  2017~2024

• 십진분류

  KDC 526 DDC 634