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Modulating the Biomechanical Culture Environment for Improved Differentiation of Human Mesenchymal Stem Cells

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    강원대학교 산림과학연구소 학술대회 바로가기
  • 통권
    KNU IFS 2018 Annual International Symposium of Institute of Forest Science (2018.09)바로가기
  • 페이지
    pp.7-7
  • 저자
    Dinesh Kumar Patel, Yu-Ri Seo, Ki-Taek Lim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A450136

원문정보

초록

영어
Human mesenchymal stem cells (hMSCs) are critical for numerous groundbreaking therapies in the field of regenerative medicine. Nanoscale topography of artificial substrates can greatly influence the fate of stem cells including adhesion, proliferation, and differentiation. Thus the design and manipulation of reduced graphene oxide (rGO)-based nanosheets and its electrical properties are of great importance to realize graphene-based electronics as a strategy in stem cells and tissue engineering applications. In this report, we propose that electro-conductive graphene oxide nanosheets with pulsed electromagnetic fields (PEMFs) are an efficient platform for modulating and enhancing structure and function of hMSCs. Using a self-assembly method, we successfully coated graphene oxide (GO) on glass for fabricating GO nanocomposite and had tunable electrical conductivity of graphene oxide sheets. The hMSCs grown on the rGO films under PEMFs showed increased adhesion, indicated by a large number of focal adhesions, and higher mineralization markers of the extracellular matrix and their osteogenic differentiation in a controlled manner through vinculin, vimentin, osteopontin (OPN), fibronectin, calmodulin (caM) expressions, and differential hierarchical clustering by microarray in hMSCs. Here we show that rGO with PEMFs as a graphene-based cell stimulator provides a promising biocompatible nanocomposite that does not hamper the proliferation of hMSCs and accelerates their specific differentiation into bone cells. Our bottom-up biomechatronic approach of tuning the rGO-sheet properties provides a path to a broad new class of graphene-based materials and their use in a variety of applications.

키워드

Graphene Reduced Graphene Oxide Electro-conduction Electromagnetic Fields Stem Cells

저자

  • Dinesh Kumar Patel [ Dept. of Biosystems Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ]
  • Yu-Ri Seo [ Dept. of Biosystems Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ]
  • Ki-Taek Lim [ Dept. of Biosystems Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    강원대학교 산림과학연구소 학술대회
  • 간기
    부정기
  • 수록기간
    2017~2024
  • 십진분류
    KDC 526 DDC 634

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