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Efficient and rapid co-fermentation of mixed sugars by engineered yeast

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    강원대학교 산림과학연구소 학술대회 바로가기
  • 통권
    KNU IFS 2018 Annual International Symposium of Institute of Forest Science (2018.09)바로가기
  • 페이지
    pp.6-6
  • 저자
    Suk-Jin, Ha, Jae-Bum Park, Jin-Seong Kim, Deok-Ho Kwon, Saet-Byeol Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A450135

원문정보

초록

영어
Cellulosic biomass is a renewable source for biofuel productions as a non-edible biomass. In order to utilize cellulosic biomass efficiently, co-fermentation of mixed sugars (glucose and xylose) is required. Kluyveromyces marxianus is well known thermotolerent yeast which is capable of growing at 45℃ using glucose as a carbon source. However, xylose utilization is not efficient as a sole carbon source by K. marxianus. For improved xylose utilization, the mutant KmXYL1 gene coded for xylose reductase (XR) and the mutant KmXYL2 gene coded for xylitol dehydrogenase (XDH) were obtained from K. marxianus 36907-FMEL1 and K. marxianus 17694-DH1, respectively. The mutant XR from K. marxianus 36907-FMEL1 exhibited around 2 fold higher enzymatic activity that the wild type XR and the mutant XDH from K. marxianus 17694-DH1 exhibited around 65% higher enzymatic activity than the wild type XDH. When the mutant KmXYL1 was overexpressed, xylose consumption and xylitol production rates were highly increased. And the mutant KmXYL1 and KmXYL2 were overexpressed together, xylose consumption and ethanol production rates were highly increased.

키워드

cellulosic biomass Kluyveronmyce marxianus biofuel xylose reductase xylitol dehydrogenas

저자

  • Suk-Jin, Ha [ Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ] Corresponding Author
  • Jae-Bum Park [ Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ]
  • Jin-Seong Kim [ Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ]
  • Deok-Ho Kwon [ Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ]
  • Saet-Byeol Kim [ Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    강원대학교 산림과학연구소 학술대회
  • 간기
    부정기
  • 수록기간
    2017~2024
  • 십진분류
    KDC 526 DDC 634

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