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Antimicrobial and neurotoxic activities of novel peptides in Korean spider venom

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    강원대학교 산림과학연구소 학술대회 바로가기
  • 통권
    KNU IFS 2017 Annual International Symposium of Institute of Forest Science (2017.11)바로가기
  • 페이지
    pp.25-25
  • 저자
    In-Wook Hwang, Min-Kyung Shin, Jung-Suk Sung
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A450048

원문정보

초록

영어
Spiders are divided into two classes, wandering and web-building spiders, according to their ecological characteristics, and thus their living environment and the process of collecting preys are distinct. In this study, we investigated the differences in functional properties of spider venom according to the ecological characteristics of wandering spider and web-building spider, respectively. We determined growth inhibition effects of selected spiders using gram-positive (B. cereus) and gram-negative (E. coli) bacteria. In addition, the activation or inhibition of ion channel (Ca2+ channel, Na+ channel, and nicotinic acetylcholine receptor) were confirmed by measuring intracellular Ca2+ concentration in SH-SY5Y cells using the selected spider venoms. Analysis of antibacterial and neurotoxic activities of the venom indicated that wandering spiders are more effective for antibacterial function compared to web-building spiders, while web-building spiders are more cellular neurotoxic than wandering spiders. Furthermore, the analysis of sequences of expressed RNA in the venom gland was performed by using Argiope bruennichi to identify the peptides containing antimicrobial and neurotoxic activities. We evaluated the effect of the identified venom peptides in growth inhibition of fungi (F. oxysporum) and the activation or inhibition on the Ca2+ channel. Our results showed that two novel venom peptides have antifungal activity, which exhibit the function to enhance the cell membrane permeability of F. oxysporum. Moreover, we verified two distinct venom peptides that significantly inhibited Ca2+ channels. Antimicrobial activity and the efficacy in inactivating Ca2+ channel, which were found in the peptides specific for the venom derived from Argiope bruennichi, can be utilized in development of natural antifungal agents as well as anesthetic or painkiller related to its neurotoxicity.

키워드

spider venom ecological characteristics antimicrobial neurotoxicity RNA sequencing

저자

  • In-Wook Hwang [ Department of Life Science, Dongguk University-Seoul ] Corresponding Author
  • Min-Kyung Shin [ Department of Life Science, Dongguk University-Seoul ]
  • Jung-Suk Sung [ Department of Life Science, Dongguk University-Seoul ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    강원대학교 산림과학연구소 학술대회
  • 간기
    부정기
  • 수록기간
    2017~2024
  • 십진분류
    KDC 526 DDC 634

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