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Application of functionality involved in venom derived from Korean spiders

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    강원대학교 산림과학연구소 학술대회 바로가기
  • 통권
    KNU IFS 2017 Annual International Symposium of Institute of Forest Science (2017.11)바로가기
  • 페이지
    pp.24-24
  • 저자
    In-Wook Hwang, Min-Kyoung Shin, Jung-Suk Sung
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A450047

원문정보

초록

영어
Venom of spiders is composed of various peptides, which have distinct physiological characteristics. We collected venom derived from domestic indigenous spiders including Argiope bruennichi and Pardosa astrigera, and proceeded evaluation for antimicrobial and anticancer activities. Growth inhibitory effects of spider venom against gram-positive (B. cereus and S. aureus), gram-negative (E. carotovora and E. coli) bacteria, and fungi (F. oxysporum) was determined, respectively. Analysis of cell membrane permeability showed that the venom from both spiders possess significant antimicrobial activity via increasing bacterial and fungal cell permeability. Cytotoxic effects of the spider venom were performed on normal cell line (hAD-MSCs) and cancer cell lines (HepG2, AGS, HeLa, and H460 cells). We found anti-proliferative activity of venoms against cancer cells, while the up-regulation of apoptosis related genes appeared to be correlated with such cytotoxic effects against cancer cell lines. De novo peptide sequencing was conducted, and partial sequences of venom peptides were verified. We found that the identified peptides are novel, but share homology with toxin related peptides reported in Araneae database. We selected domestic indigenous spider, A. bruennichi, and performed RNA sequencing to identify the expression of the venom specific genes. We finally identified five novel toxin-like peptides, predicted by utilizing the homology and its structural characterization analysis of acquired genes. These results imply the feasibility in developing antimicrobial and anticancer agents by utilizing novel peptide derived from spider venoms.

키워드

spider venom antimicrobial anticancer De novo peptide sequencing RNA sequencing

저자

  • In-Wook Hwang [ Department of Life Science, Dongguk University-Seoul ]
  • Min-Kyoung Shin [ Department of Life Science, Dongguk University-Seoul ]
  • Jung-Suk Sung [ Department of Life Science, Dongguk University-Seoul ] Corresponding Author

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    강원대학교 산림과학연구소 학술대회
  • 간기
    부정기
  • 수록기간
    2017~2024
  • 십진분류
    KDC 526 DDC 634

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