Earticle

초록

영어

Background: The small intestine plays a crucial role in animals in maintaining homeostasis as well as a series of physiological events such as nutrient uptake and immune function to improve productivity. Research on intestinal organoids has recently garnered interest, aiming to study various functions of the intestinal epithelium as a potential alternative to an in vivo system. These technologies have created new possibilities and opportunities for substituting animals for testing with an in vitro model. Methods: Here, we report the establishment and characterisation of intestinal organoids derived from jejunum tissues of adult pigs. Intestinal crypts, including intestinal stem cells from the jejunum tissue of adult pigs (10 months old), were sequentially isolated and cultivated over several passages without losing their proliferation and differentiation using the scaffold-based and three-dimensional method, which indicated the recapitulating capacity. Results: Porcine jejunum-derived intestinal organoids showed the specific expression of several genes related to intestinal stem cells and the epithelium. Furthermore, they showed high permeability when exposed to FITC-dextran 4 kDa, representing a barrier function similar to that of in vivo tissues. Collectively, these results demonstrate the efficient cultivation and characteristics of porcine jejunum-derived intestinal organoids. Conclusions: In this study, using a 3D culture system, we successfully established porcine jejunum-derived intestinal organoids. They show potential for various applications, such as for nutrient absorption as an in vitro model of the intestinal epithelium fused with organ-on-a-chip technology to improve productivity in animal biotechnology in future studies.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Experimental design and animals
Intestinal crypt isolation and 3D cultivation
Intestinal organoid growth, passage, and cryopreservation
RNA isolation
Quantitative real time polymerase chain reaction (RTPCR)
Library preparation and sequencing
Data processing and analysis
Differential gene expression analysis
Immunocytochemistry
Epithelial barrier permeability assay using FITCdextran
Statistical analysis
RESULTS
Growth performance and long-term maintenance ofporcine jejunum-derived intestinal organoids
Gene expression profiling of porcine jejunum-derived intestinal organoids
Characterisation and paracellular permeability of porcine jejunum-derived intestinal organoids
DISCUSSION
CONCLUSION
REFERENCES

키워드

저자

• Bo Ram Lee [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea ] Corresponding Author
• Sun A Ock [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea ]
• Mi Ryung Park [ Animal Genetic Resources Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, Korea ]
• Min Gook Lee [ Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea ]
• Sung June Byun [ Poultry Research Institute, National Institute of Animal Science, Rural Development Administration, Pyeongchang 25342, Korea ]

참고문헌

발행기관

간행물

관심저널 등록

• 간행물명

  Journal of Animal Reproduction and Biotechnology

• 간기

  계간

• pISSN

  2671-4639

• eISSN

  2671-4663

• 수록기간

  2019~2026

• 십진분류

  KDC 527 DDC 636